Monitoring of antimicrobial drug chloramphenicol release from electrospun nano-and microfiber mats using UV imaging and bacterial bioreporters
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Monitoring of antimicrobial drug chloramphenicol release from electrospun nano-and microfiber mats using UV imaging and bacterial bioreporters. / Preem, Liis; Bock, Frederik; Hinnu, Mariliis; Putrinš, Marta; Sagor, Kadi; Tenson, Tanel; Meos, Andres; Østergaard, Jesper; Kogermann, Karin.
In: Pharmaceutics, Vol. 11, No. 9, 487, 2019.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Monitoring of antimicrobial drug chloramphenicol release from electrospun nano-and microfiber mats using UV imaging and bacterial bioreporters
AU - Preem, Liis
AU - Bock, Frederik
AU - Hinnu, Mariliis
AU - Putrinš, Marta
AU - Sagor, Kadi
AU - Tenson, Tanel
AU - Meos, Andres
AU - Østergaard, Jesper
AU - Kogermann, Karin
PY - 2019
Y1 - 2019
N2 - New strategies are continuously sought for the treatment of skin and wound infections due to increased problems with non-healing wounds. Electrospun nanofiber mats with antibacterial agents as drug delivery systems provide opportunities for the eradication of bacterial infections as well as wound healing. Antibacterial activities of such mats are directly linked with their drug release behavior. Traditional pharmacopoeial drug release testing settings are not always suitable for analyzing the release behavior of fiber mats intended for the local drug delivery. We tested and compared different drug release model systems for the previously characterized electrospun chloramphenicol (CAM)-loaded nanofiber (polycaprolactone (PCL)) and microfiber (PCL in combination with polyethylene oxide) mats with different drug release profiles. Drug release into buffer solution and hydrogel was investigated and drug concentration was determined using either high-performance liquid chromatography, ultraviolet-visible spectrophotometry, or ultraviolet (UV) imaging. The CAM release and its antibacterial effects in disc diffusion assay were assessed by bacterial bioreporters. All tested model systems enabled to study the drug release from electrospun mats. It was found that the release into buffer solution showed larger differences in the drug release rate between differently designed mats compared to the hydrogel release tests. The UV imaging method provided an insight into the interactions with an agarose hydrogel mimicking wound tissue, thus giving us information about early drug release from the mat. Bacterial bioreporters showed clear correlations between the drug release into gel and antibacterial activity of the electrospun CAM-loaded mats.
AB - New strategies are continuously sought for the treatment of skin and wound infections due to increased problems with non-healing wounds. Electrospun nanofiber mats with antibacterial agents as drug delivery systems provide opportunities for the eradication of bacterial infections as well as wound healing. Antibacterial activities of such mats are directly linked with their drug release behavior. Traditional pharmacopoeial drug release testing settings are not always suitable for analyzing the release behavior of fiber mats intended for the local drug delivery. We tested and compared different drug release model systems for the previously characterized electrospun chloramphenicol (CAM)-loaded nanofiber (polycaprolactone (PCL)) and microfiber (PCL in combination with polyethylene oxide) mats with different drug release profiles. Drug release into buffer solution and hydrogel was investigated and drug concentration was determined using either high-performance liquid chromatography, ultraviolet-visible spectrophotometry, or ultraviolet (UV) imaging. The CAM release and its antibacterial effects in disc diffusion assay were assessed by bacterial bioreporters. All tested model systems enabled to study the drug release from electrospun mats. It was found that the release into buffer solution showed larger differences in the drug release rate between differently designed mats compared to the hydrogel release tests. The UV imaging method provided an insight into the interactions with an agarose hydrogel mimicking wound tissue, thus giving us information about early drug release from the mat. Bacterial bioreporters showed clear correlations between the drug release into gel and antibacterial activity of the electrospun CAM-loaded mats.
KW - Antibacterial activity
KW - Bacterial bioreporters
KW - Drug release
KW - Electrospinning
KW - Microfibers
KW - Nanofibers
KW - UV imaging
U2 - 10.3390/pharmaceutics11090487
DO - 10.3390/pharmaceutics11090487
M3 - Journal article
C2 - 31546922
AN - SCOPUS:85073570163
VL - 11
JO - Pharmaceutics
JF - Pharmaceutics
SN - 1999-4923
IS - 9
M1 - 487
ER -
ID: 242297743