Microfluidics-based self-assembly of peptide-loaded microgels: Effect of three dimensional (3D) printed micromixer design

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Microfluidics-based self-assembly of peptide-loaded microgels : Effect of three dimensional (3D) printed micromixer design. / Borro, Bruno C; Bohr, Adam; Bucciarelli, Saskia; Boetker, Johan P; Foged, Camilla; Rantanen, Jukka; Malmsten, Martin.

In: Journal of Colloid and Interface Science, Vol. 538, 07.03.2019, p. 559-568.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Borro, BC, Bohr, A, Bucciarelli, S, Boetker, JP, Foged, C, Rantanen, J & Malmsten, M 2019, 'Microfluidics-based self-assembly of peptide-loaded microgels: Effect of three dimensional (3D) printed micromixer design', Journal of Colloid and Interface Science, vol. 538, pp. 559-568. https://doi.org/10.1016/j.jcis.2018.12.010

APA

Borro, B. C., Bohr, A., Bucciarelli, S., Boetker, J. P., Foged, C., Rantanen, J., & Malmsten, M. (2019). Microfluidics-based self-assembly of peptide-loaded microgels: Effect of three dimensional (3D) printed micromixer design. Journal of Colloid and Interface Science, 538, 559-568. https://doi.org/10.1016/j.jcis.2018.12.010

Vancouver

Borro BC, Bohr A, Bucciarelli S, Boetker JP, Foged C, Rantanen J et al. Microfluidics-based self-assembly of peptide-loaded microgels: Effect of three dimensional (3D) printed micromixer design. Journal of Colloid and Interface Science. 2019 Mar 7;538:559-568. https://doi.org/10.1016/j.jcis.2018.12.010

Author

Borro, Bruno C ; Bohr, Adam ; Bucciarelli, Saskia ; Boetker, Johan P ; Foged, Camilla ; Rantanen, Jukka ; Malmsten, Martin. / Microfluidics-based self-assembly of peptide-loaded microgels : Effect of three dimensional (3D) printed micromixer design. In: Journal of Colloid and Interface Science. 2019 ; Vol. 538. pp. 559-568.

Bibtex

@article{efe7bd5e05c94ade912bf0f86ae53e71,
title = "Microfluidics-based self-assembly of peptide-loaded microgels: Effect of three dimensional (3D) printed micromixer design",
abstract = "In an effort to contribute to research in scalable production systems for polymeric delivery systems loaded with antimicrobial peptides (AMPs), we here investigate effects of hydrodynamic flow conditions on microfluidic particle generation. For this purpose, rapid prototyping using 3D printing was applied to prepare micromixers with three different geometric designs, which were used to prepare Ca2+-cross-linked alginate microgels loaded with the AMP polymyxin B in a continuous process. Based on fluid dynamic simulations, the hydrodynamic flow patterns in the micromixers were designed to be either (i) turbulent with chaotic disruption, (ii) laminar with convective mixing, or (iii) convective with microvortex formation. The physicochemical properties of the microgels prepared with these micromixers were characterized by photon correlation spectroscopy, laser-Doppler micro-electrophoresis, small-angle x-ray scattering, and ellipsometry. The particle size and compactness were found to depend on the micromixer geometry: From such studies, particle size and compactness were found to depend on micromixer geometry, the smallest and most compact particles were obtained by preparation involving microvortex flows, while larger and more diffuse microgels were formed upon laminar mixing. Polymyxin B was found to be localized in the particle interior and to cause particle growth with increasing peptide loading. Ca2+-induced cross-linking of alginate, in turn, results in particle contraction. The peptide encapsulation efficiency was found to be higher than 80% for all investigated micromixer designs; the highest encapsulation efficiency observed for the smallest particles generated by microvortex-mediated self-assembly. Ellipsometry results for surface-immobilized microgels, as well as results on peptide encapsulation, demonstrated electrolyte-induced peptide release. Taken together, these findings demonstrate that rapid prototyping of microfluidics using 3D-printed micromixers offers promises for continuous manufacturing of AMP-loaded microgels. Although the micromixer combining turbulent flow and microvortexes was demonstrated to be the most efficient, all three micromixer designs were found to mediate self-assembly of small microgels displaying efficient peptide encapsulation. This demonstrates the robustness of employing 3D-printed micromixers for microfluidic assembly of AMP-loaded microgels during continuous production.",
author = "Borro, {Bruno C} and Adam Bohr and Saskia Bucciarelli and Boetker, {Johan P} and Camilla Foged and Jukka Rantanen and Martin Malmsten",
note = "Copyright {\textcopyright} 2018 Elsevier Inc. All rights reserved.",
year = "2019",
month = mar,
day = "7",
doi = "10.1016/j.jcis.2018.12.010",
language = "English",
volume = "538",
pages = "559--568",
journal = "Journal of Colloid and Interface Science",
issn = "0021-9797",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Microfluidics-based self-assembly of peptide-loaded microgels

T2 - Effect of three dimensional (3D) printed micromixer design

AU - Borro, Bruno C

AU - Bohr, Adam

AU - Bucciarelli, Saskia

AU - Boetker, Johan P

AU - Foged, Camilla

AU - Rantanen, Jukka

AU - Malmsten, Martin

N1 - Copyright © 2018 Elsevier Inc. All rights reserved.

PY - 2019/3/7

Y1 - 2019/3/7

N2 - In an effort to contribute to research in scalable production systems for polymeric delivery systems loaded with antimicrobial peptides (AMPs), we here investigate effects of hydrodynamic flow conditions on microfluidic particle generation. For this purpose, rapid prototyping using 3D printing was applied to prepare micromixers with three different geometric designs, which were used to prepare Ca2+-cross-linked alginate microgels loaded with the AMP polymyxin B in a continuous process. Based on fluid dynamic simulations, the hydrodynamic flow patterns in the micromixers were designed to be either (i) turbulent with chaotic disruption, (ii) laminar with convective mixing, or (iii) convective with microvortex formation. The physicochemical properties of the microgels prepared with these micromixers were characterized by photon correlation spectroscopy, laser-Doppler micro-electrophoresis, small-angle x-ray scattering, and ellipsometry. The particle size and compactness were found to depend on the micromixer geometry: From such studies, particle size and compactness were found to depend on micromixer geometry, the smallest and most compact particles were obtained by preparation involving microvortex flows, while larger and more diffuse microgels were formed upon laminar mixing. Polymyxin B was found to be localized in the particle interior and to cause particle growth with increasing peptide loading. Ca2+-induced cross-linking of alginate, in turn, results in particle contraction. The peptide encapsulation efficiency was found to be higher than 80% for all investigated micromixer designs; the highest encapsulation efficiency observed for the smallest particles generated by microvortex-mediated self-assembly. Ellipsometry results for surface-immobilized microgels, as well as results on peptide encapsulation, demonstrated electrolyte-induced peptide release. Taken together, these findings demonstrate that rapid prototyping of microfluidics using 3D-printed micromixers offers promises for continuous manufacturing of AMP-loaded microgels. Although the micromixer combining turbulent flow and microvortexes was demonstrated to be the most efficient, all three micromixer designs were found to mediate self-assembly of small microgels displaying efficient peptide encapsulation. This demonstrates the robustness of employing 3D-printed micromixers for microfluidic assembly of AMP-loaded microgels during continuous production.

AB - In an effort to contribute to research in scalable production systems for polymeric delivery systems loaded with antimicrobial peptides (AMPs), we here investigate effects of hydrodynamic flow conditions on microfluidic particle generation. For this purpose, rapid prototyping using 3D printing was applied to prepare micromixers with three different geometric designs, which were used to prepare Ca2+-cross-linked alginate microgels loaded with the AMP polymyxin B in a continuous process. Based on fluid dynamic simulations, the hydrodynamic flow patterns in the micromixers were designed to be either (i) turbulent with chaotic disruption, (ii) laminar with convective mixing, or (iii) convective with microvortex formation. The physicochemical properties of the microgels prepared with these micromixers were characterized by photon correlation spectroscopy, laser-Doppler micro-electrophoresis, small-angle x-ray scattering, and ellipsometry. The particle size and compactness were found to depend on the micromixer geometry: From such studies, particle size and compactness were found to depend on micromixer geometry, the smallest and most compact particles were obtained by preparation involving microvortex flows, while larger and more diffuse microgels were formed upon laminar mixing. Polymyxin B was found to be localized in the particle interior and to cause particle growth with increasing peptide loading. Ca2+-induced cross-linking of alginate, in turn, results in particle contraction. The peptide encapsulation efficiency was found to be higher than 80% for all investigated micromixer designs; the highest encapsulation efficiency observed for the smallest particles generated by microvortex-mediated self-assembly. Ellipsometry results for surface-immobilized microgels, as well as results on peptide encapsulation, demonstrated electrolyte-induced peptide release. Taken together, these findings demonstrate that rapid prototyping of microfluidics using 3D-printed micromixers offers promises for continuous manufacturing of AMP-loaded microgels. Although the micromixer combining turbulent flow and microvortexes was demonstrated to be the most efficient, all three micromixer designs were found to mediate self-assembly of small microgels displaying efficient peptide encapsulation. This demonstrates the robustness of employing 3D-printed micromixers for microfluidic assembly of AMP-loaded microgels during continuous production.

U2 - 10.1016/j.jcis.2018.12.010

DO - 10.1016/j.jcis.2018.12.010

M3 - Journal article

C2 - 30551068

VL - 538

SP - 559

EP - 568

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

ER -

ID: 210152760