TY - JOUR

T1 - Membrane interactions in drug delivery

T2 - Model cell membranes and orthogonal techniques

AU - Bunea, Ada-Ioana

AU - Harloff-Helleberg, Stine

AU - Taboryski, Rafael

AU - Nielsen, Hanne Morck

PY - 2020

Y1 - 2020

N2 - To generate the desired effect in the human body, the active pharmaceutical ingredient usually needs to interact with a receptor located on the cell membrane or inside the cell. Thus, understanding membrane interactions is of great importance when it comes to the development and testing of new drug molecules or new drug delivery systems. Nowadays, there is a tremendous selection of both model cell membranes and of techniques that can be used to characterize interactions between selected model cell membranes and a drug molecule, an excipient, or a drug delivery system. Having such a wide selection of model cell membranes and techniques available makes it sometimes challenging to select the optimal combination for a specific study. Furthermore, it is difficult to compare results obtained using different model cell membranes and techniques, and not all in vitro studies translate as well to an estimation of the in vivo biological activity or understanding of mode of action. This review provides an overview of the available lipid bilayer-based model cell membranes and of the most widely employed techniques for studying membrane interactions. Finally, the need for employing complimentary characterization techniques in order to acquire more reliable and in-depth information is highlighted. (C) 2020 Published by Elsevier B.V.

AB - To generate the desired effect in the human body, the active pharmaceutical ingredient usually needs to interact with a receptor located on the cell membrane or inside the cell. Thus, understanding membrane interactions is of great importance when it comes to the development and testing of new drug molecules or new drug delivery systems. Nowadays, there is a tremendous selection of both model cell membranes and of techniques that can be used to characterize interactions between selected model cell membranes and a drug molecule, an excipient, or a drug delivery system. Having such a wide selection of model cell membranes and techniques available makes it sometimes challenging to select the optimal combination for a specific study. Furthermore, it is difficult to compare results obtained using different model cell membranes and techniques, and not all in vitro studies translate as well to an estimation of the in vivo biological activity or understanding of mode of action. This review provides an overview of the available lipid bilayer-based model cell membranes and of the most widely employed techniques for studying membrane interactions. Finally, the need for employing complimentary characterization techniques in order to acquire more reliable and in-depth information is highlighted. (C) 2020 Published by Elsevier B.V.

KW - Model cell membrane

KW - membrane interaction

KW - lipid bilayer

KW - drug delivery

KW - drug testing

KW - SUPPORTED LIPID-BILAYERS

KW - MOLECULAR-DYNAMICS SIMULATIONS

KW - SURFACE-PLASMON RESONANCE

KW - CRYOELECTRON MICROSCOPY STRUCTURE

KW - QUARTZ-CRYSTAL MICROBALANCE

KW - BIOLOGICAL-MEMBRANES

KW - PATCH-CLAMP

KW - ANTIMICROBIAL PEPTIDES

KW - PHOSPHOLIPID-VESICLES

KW - GOLD NANOPARTICLES

U2 - 10.1016/j.cis.2020.102177

DO - 10.1016/j.cis.2020.102177

M3 - Review

C2 - 32417568

VL - 281

JO - Advances in Colloid and Interface Science

JF - Advances in Colloid and Interface Science

SN - 0001-8686

M1 - 102177

ER -