Mechanism of Action of Lung Damage Caused by a Nanofilm Spray Product

Research output: Contribution to journalJournal articlepeer-review

Standard

Mechanism of Action of Lung Damage Caused by a Nanofilm Spray Product. / Larsen, Søren T.; Dallot, Constantin; Larsen, Susan W; Rose, Fabrice; Poulsen, Steen Seier; Nørgaard, Asger W; Hansen, Jitka S; Sørli, Jorid B; Nielsen, Gunnar D; Foged, Camilla.

In: Toxicological Sciences, Vol. 140, No. 2, 25.05.2014, p. 436-444.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Larsen, ST, Dallot, C, Larsen, SW, Rose, F, Poulsen, SS, Nørgaard, AW, Hansen, JS, Sørli, JB, Nielsen, GD & Foged, C 2014, 'Mechanism of Action of Lung Damage Caused by a Nanofilm Spray Product', Toxicological Sciences, vol. 140, no. 2, pp. 436-444. https://doi.org/10.1093/toxsci/kfu098

APA

Larsen, S. T., Dallot, C., Larsen, S. W., Rose, F., Poulsen, S. S., Nørgaard, A. W., Hansen, J. S., Sørli, J. B., Nielsen, G. D., & Foged, C. (2014). Mechanism of Action of Lung Damage Caused by a Nanofilm Spray Product. Toxicological Sciences, 140(2), 436-444. https://doi.org/10.1093/toxsci/kfu098

Vancouver

Larsen ST, Dallot C, Larsen SW, Rose F, Poulsen SS, Nørgaard AW et al. Mechanism of Action of Lung Damage Caused by a Nanofilm Spray Product. Toxicological Sciences. 2014 May 25;140(2):436-444. https://doi.org/10.1093/toxsci/kfu098

Author

Larsen, Søren T. ; Dallot, Constantin ; Larsen, Susan W ; Rose, Fabrice ; Poulsen, Steen Seier ; Nørgaard, Asger W ; Hansen, Jitka S ; Sørli, Jorid B ; Nielsen, Gunnar D ; Foged, Camilla. / Mechanism of Action of Lung Damage Caused by a Nanofilm Spray Product. In: Toxicological Sciences. 2014 ; Vol. 140, No. 2. pp. 436-444.

Bibtex

@article{47dd6c52833f4dfc975e4c67476c7169,
title = "Mechanism of Action of Lung Damage Caused by a Nanofilm Spray Product",
abstract = "Inhalation of waterproofing spray products has on several occasions caused lung damage, which in some cases was fatal. The present study aims to elucidate the mechanism of action of a nanofilm spray product, which has been shown to possess unusual toxic effects, including an extremely steep concentration-effect curve. The nanofilm product is intended for application on non-absorbing flooring materials and contains perfluorosiloxane as the active film-forming component. The toxicological effects and their underlying mechanisms of this product were studied using a mouse inhalation model, by in vitro techniques and by identification of the binding interaction. Inhalation of the aerosolized product gave rise to increased airway resistance in the mice, as evident from the decreased expiratory flow rate. The toxic effect of the waterproofing spray product included interaction with the pulmonary surfactants. More specifically, the active film-forming components in the spray product, perfluorinated siloxanes, inhibited the function of the lung surfactant due to non-covalent interaction with surfactant protein B, a component which is crucial for the stability and persistence of the lung surfactant film during respiration. The active film-forming component used in the present spray product is also found in several other products on the market. Hence, it may be expected that these products may have a toxicity similar to the waterproofing product studied here. Elucidation of the toxicological mechanism and identification of toxicological targets are important to perform rational and cost-effective toxicological studies. Thus, because the pulmonary surfactant system appears to be an important toxicological target for waterproofing spray products, study of surfactant inhibition could be included in toxicological assessment of this group of consumer products. Key Words: Waterproofing spay product; lung toxicity; inhalation; pulmonary surfactant.",
author = "Larsen, {S{\o}ren T.} and Constantin Dallot and Larsen, {Susan W} and Fabrice Rose and Poulsen, {Steen Seier} and N{\o}rgaard, {Asger W} and Hansen, {Jitka S} and S{\o}rli, {Jorid B} and Nielsen, {Gunnar D} and Camilla Foged",
note = "{\textcopyright} The Author(s) 2014. Published by Oxford University Press on behalf of Toxicological Sciences.",
year = "2014",
month = may,
day = "25",
doi = "10.1093/toxsci/kfu098",
language = "English",
volume = "140",
pages = "436--444",
journal = "Toxicological Sciences",
issn = "1096-6080",
publisher = "Oxford University Press",
number = "2",

}

RIS

TY - JOUR

T1 - Mechanism of Action of Lung Damage Caused by a Nanofilm Spray Product

AU - Larsen, Søren T.

AU - Dallot, Constantin

AU - Larsen, Susan W

AU - Rose, Fabrice

AU - Poulsen, Steen Seier

AU - Nørgaard, Asger W

AU - Hansen, Jitka S

AU - Sørli, Jorid B

AU - Nielsen, Gunnar D

AU - Foged, Camilla

N1 - © The Author(s) 2014. Published by Oxford University Press on behalf of Toxicological Sciences.

PY - 2014/5/25

Y1 - 2014/5/25

N2 - Inhalation of waterproofing spray products has on several occasions caused lung damage, which in some cases was fatal. The present study aims to elucidate the mechanism of action of a nanofilm spray product, which has been shown to possess unusual toxic effects, including an extremely steep concentration-effect curve. The nanofilm product is intended for application on non-absorbing flooring materials and contains perfluorosiloxane as the active film-forming component. The toxicological effects and their underlying mechanisms of this product were studied using a mouse inhalation model, by in vitro techniques and by identification of the binding interaction. Inhalation of the aerosolized product gave rise to increased airway resistance in the mice, as evident from the decreased expiratory flow rate. The toxic effect of the waterproofing spray product included interaction with the pulmonary surfactants. More specifically, the active film-forming components in the spray product, perfluorinated siloxanes, inhibited the function of the lung surfactant due to non-covalent interaction with surfactant protein B, a component which is crucial for the stability and persistence of the lung surfactant film during respiration. The active film-forming component used in the present spray product is also found in several other products on the market. Hence, it may be expected that these products may have a toxicity similar to the waterproofing product studied here. Elucidation of the toxicological mechanism and identification of toxicological targets are important to perform rational and cost-effective toxicological studies. Thus, because the pulmonary surfactant system appears to be an important toxicological target for waterproofing spray products, study of surfactant inhibition could be included in toxicological assessment of this group of consumer products. Key Words: Waterproofing spay product; lung toxicity; inhalation; pulmonary surfactant.

AB - Inhalation of waterproofing spray products has on several occasions caused lung damage, which in some cases was fatal. The present study aims to elucidate the mechanism of action of a nanofilm spray product, which has been shown to possess unusual toxic effects, including an extremely steep concentration-effect curve. The nanofilm product is intended for application on non-absorbing flooring materials and contains perfluorosiloxane as the active film-forming component. The toxicological effects and their underlying mechanisms of this product were studied using a mouse inhalation model, by in vitro techniques and by identification of the binding interaction. Inhalation of the aerosolized product gave rise to increased airway resistance in the mice, as evident from the decreased expiratory flow rate. The toxic effect of the waterproofing spray product included interaction with the pulmonary surfactants. More specifically, the active film-forming components in the spray product, perfluorinated siloxanes, inhibited the function of the lung surfactant due to non-covalent interaction with surfactant protein B, a component which is crucial for the stability and persistence of the lung surfactant film during respiration. The active film-forming component used in the present spray product is also found in several other products on the market. Hence, it may be expected that these products may have a toxicity similar to the waterproofing product studied here. Elucidation of the toxicological mechanism and identification of toxicological targets are important to perform rational and cost-effective toxicological studies. Thus, because the pulmonary surfactant system appears to be an important toxicological target for waterproofing spray products, study of surfactant inhibition could be included in toxicological assessment of this group of consumer products. Key Words: Waterproofing spay product; lung toxicity; inhalation; pulmonary surfactant.

U2 - 10.1093/toxsci/kfu098

DO - 10.1093/toxsci/kfu098

M3 - Journal article

C2 - 24863969

VL - 140

SP - 436

EP - 444

JO - Toxicological Sciences

JF - Toxicological Sciences

SN - 1096-6080

IS - 2

ER -

ID: 113181110