Liposomal delivery of antigen to human dendritic cells
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Liposomal delivery of antigen to human dendritic cells. / Copland, Melissa J.; Baird, Margaret A.; Rades, Thomas; McKenzie, Judith L.; Becker, Bernd; Reck, Folkert; Tyler, Peter C.; Davies, Nigel M.
In: Vaccine, Vol. 21, No. 9-10, 14.02.2003, p. 883-890.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Liposomal delivery of antigen to human dendritic cells
AU - Copland, Melissa J.
AU - Baird, Margaret A.
AU - Rades, Thomas
AU - McKenzie, Judith L.
AU - Becker, Bernd
AU - Reck, Folkert
AU - Tyler, Peter C.
AU - Davies, Nigel M.
N1 - Funding Information: The authors gratefully acknowledge Otago Research Grants for provision of funding for this work.
PY - 2003/2/14
Y1 - 2003/2/14
N2 - This study investigated whether formulation of antigen in mannosylated liposomes enhanced uptake and activation of dendritic cells (DC) and increased the ability of DC to induce primed T cell proliferation compared to formulation of antigen in unmodified liposomes or in solution. Immature human DC were generated from peripheral blood monocytes cultured with GM-CSF and IL-4. Uptake of antigen by DC and the degree of expression of the cell surface markers MHC class II, CD80, CD86 and the DC maturation marker CD83, was investigated by flow cytometry following incubation with liposomes or solution containing FITC-conjugated antigen. Exposure to liposomes containing FITC-ovalbumin resulted in enhanced expression of cell surface markers when compared to exposure to antigen in solution. Expression was highest following exposure to mannosylated liposomes. Mannosylated liposomes containing tetanus toxoid (TT) stimulated primed T cell proliferation more effectively than TT-neutral liposomes or TT-solution. This work suggests that mannosylated liposomes provide a versatile delivery vehicle for initiating enhanced immune responses to encapsulated peptide or protein vaccines.
AB - This study investigated whether formulation of antigen in mannosylated liposomes enhanced uptake and activation of dendritic cells (DC) and increased the ability of DC to induce primed T cell proliferation compared to formulation of antigen in unmodified liposomes or in solution. Immature human DC were generated from peripheral blood monocytes cultured with GM-CSF and IL-4. Uptake of antigen by DC and the degree of expression of the cell surface markers MHC class II, CD80, CD86 and the DC maturation marker CD83, was investigated by flow cytometry following incubation with liposomes or solution containing FITC-conjugated antigen. Exposure to liposomes containing FITC-ovalbumin resulted in enhanced expression of cell surface markers when compared to exposure to antigen in solution. Expression was highest following exposure to mannosylated liposomes. Mannosylated liposomes containing tetanus toxoid (TT) stimulated primed T cell proliferation more effectively than TT-neutral liposomes or TT-solution. This work suggests that mannosylated liposomes provide a versatile delivery vehicle for initiating enhanced immune responses to encapsulated peptide or protein vaccines.
KW - Dendritic cells
KW - Immunotherapy
KW - Liposomes
UR - http://www.scopus.com/inward/record.url?scp=0037435875&partnerID=8YFLogxK
U2 - 10.1016/S0264-410X(02)00536-4
DO - 10.1016/S0264-410X(02)00536-4
M3 - Journal article
C2 - 12547598
AN - SCOPUS:0037435875
VL - 21
SP - 883
EP - 890
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 9-10
ER -
ID: 299429775