In vitro models of the blood-brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use

Research output: Contribution to journal › Review › Research › peer-review

Standard

In vitro models of the blood-brain barrier : An overview of commonly used brain endothelial cell culture models and guidelines for their use. / Helms, Hans Christian Cederberg; Abbott, N Joan; Burek, Malgorzata; Cecchelli, Romeo; Couraud, Pierre-Olivier; Deli, Maria A; Förster, Carola; Galla, Hans J; Romero, Ignacio A; Shusta, Eric V; Stebbins, Matthew J; Vandenhaute, Elodie; Weksler, Babette; Brodin, Birger.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 36, No. 5, 05.2016, p. 862-90.

Research output: Contribution to journal › Review › Research › peer-review

Harvard

Helms, HCC, Abbott, NJ, Burek, M, Cecchelli, R, Couraud, P-O, Deli, MA, Förster, C, Galla, HJ, Romero, IA, Shusta, EV, Stebbins, MJ, Vandenhaute, E, Weksler, B & Brodin, B 2016, 'In vitro models of the blood-brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use', Journal of Cerebral Blood Flow and Metabolism, vol. 36, no. 5, pp. 862-90. https://doi.org/10.1177/0271678X16630991

APA

Helms, H. C. C., Abbott, N. J., Burek, M., Cecchelli, R., Couraud, P-O., Deli, M. A., Förster, C., Galla, H. J., Romero, I. A., Shusta, E. V., Stebbins, M. J., Vandenhaute, E., Weksler, B., & Brodin, B. (2016). In vitro models of the blood-brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use. Journal of Cerebral Blood Flow and Metabolism, 36(5), 862-90. https://doi.org/10.1177/0271678X16630991

Vancouver

Helms HCC, Abbott NJ, Burek M, Cecchelli R, Couraud P-O, Deli MA et al. In vitro models of the blood-brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use. Journal of Cerebral Blood Flow and Metabolism. 2016 May;36(5):862-90. https://doi.org/10.1177/0271678X16630991

Author

Helms, Hans Christian Cederberg ; Abbott, N Joan ; Burek, Malgorzata ; Cecchelli, Romeo ; Couraud, Pierre-Olivier ; Deli, Maria A ; Förster, Carola ; Galla, Hans J ; Romero, Ignacio A ; Shusta, Eric V ; Stebbins, Matthew J ; Vandenhaute, Elodie ; Weksler, Babette ; Brodin, Birger. / In vitro models of the blood-brain barrier : An overview of commonly used brain endothelial cell culture models and guidelines for their use. In: Journal of Cerebral Blood Flow and Metabolism. 2016 ; Vol. 36, No. 5. pp. 862-90.

Bibtex

@article{69457cf6443b46c1ab932cae23860d3c,
title = "In vitro models of the blood-brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use",
abstract = "The endothelial cells lining the brain capillaries separate the blood from the brain parenchyma. The endothelial monolayer of the brain capillaries serves both as a crucial interface for exchange of nutrients, gases, and metabolites between blood and brain, and as a barrier for neurotoxic components of plasma and xenobiotics. This {"}blood-brain barrier{"} function is a major hindrance for drug uptake into the brain parenchyma. Cell culture models, based on either primary cells or immortalized brain endothelial cell lines, have been developed, in order to facilitate in vitro studies of drug transport to the brain and studies of endothelial cell biology and pathophysiology. In this review, we aim to give an overview of established in vitro blood-brain barrier models with a focus on their validation regarding a set of well-established blood-brain barrier characteristics. As an ideal cell culture model of the blood-brain barrier is yet to be developed, we also aim to give an overview of the advantages and drawbacks of the different models described.",
keywords = "Journal Article, Review",
author = "Helms, {Hans Christian Cederberg} and Abbott, {N Joan} and Malgorzata Burek and Romeo Cecchelli and Pierre-Olivier Couraud and Deli, {Maria A} and Carola F{\"o}rster and Galla, {Hans J} and Romero, {Ignacio A} and Shusta, {Eric V} and Stebbins, {Matthew J} and Elodie Vandenhaute and Babette Weksler and Birger Brodin",
note = "{\textcopyright} The Author(s) 2016.",
year = "2016",
month = may,
doi = "10.1177/0271678X16630991",
language = "English",
volume = "36",
pages = "862--90",
journal = "Journal of Cerebral Blood Flow and Metabolism",
issn = "0271-678X",
publisher = "SAGE Publications",
number = "5",

}

RIS

TY - JOUR

T1 - In vitro models of the blood-brain barrier

T2 - An overview of commonly used brain endothelial cell culture models and guidelines for their use

AU - Helms, Hans Christian Cederberg

AU - Abbott, N Joan

AU - Burek, Malgorzata

AU - Cecchelli, Romeo

AU - Couraud, Pierre-Olivier

AU - Deli, Maria A

AU - Förster, Carola

AU - Galla, Hans J

AU - Romero, Ignacio A

AU - Shusta, Eric V

AU - Stebbins, Matthew J

AU - Vandenhaute, Elodie

AU - Weksler, Babette

AU - Brodin, Birger

N1 - © The Author(s) 2016.

PY - 2016/5

Y1 - 2016/5

N2 - The endothelial cells lining the brain capillaries separate the blood from the brain parenchyma. The endothelial monolayer of the brain capillaries serves both as a crucial interface for exchange of nutrients, gases, and metabolites between blood and brain, and as a barrier for neurotoxic components of plasma and xenobiotics. This "blood-brain barrier" function is a major hindrance for drug uptake into the brain parenchyma. Cell culture models, based on either primary cells or immortalized brain endothelial cell lines, have been developed, in order to facilitate in vitro studies of drug transport to the brain and studies of endothelial cell biology and pathophysiology. In this review, we aim to give an overview of established in vitro blood-brain barrier models with a focus on their validation regarding a set of well-established blood-brain barrier characteristics. As an ideal cell culture model of the blood-brain barrier is yet to be developed, we also aim to give an overview of the advantages and drawbacks of the different models described.

AB - The endothelial cells lining the brain capillaries separate the blood from the brain parenchyma. The endothelial monolayer of the brain capillaries serves both as a crucial interface for exchange of nutrients, gases, and metabolites between blood and brain, and as a barrier for neurotoxic components of plasma and xenobiotics. This "blood-brain barrier" function is a major hindrance for drug uptake into the brain parenchyma. Cell culture models, based on either primary cells or immortalized brain endothelial cell lines, have been developed, in order to facilitate in vitro studies of drug transport to the brain and studies of endothelial cell biology and pathophysiology. In this review, we aim to give an overview of established in vitro blood-brain barrier models with a focus on their validation regarding a set of well-established blood-brain barrier characteristics. As an ideal cell culture model of the blood-brain barrier is yet to be developed, we also aim to give an overview of the advantages and drawbacks of the different models described.

KW - Journal Article

KW - Review

U2 - 10.1177/0271678X16630991

DO - 10.1177/0271678X16630991

M3 - Review

C2 - 26868179

VL - 36

SP - 862

EP - 890

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 5

ER -

ID: 165673888