Investigating the effect of graphene oxide in chitosan/alginate-based foams on the release and antifungal activity of clotrimazole in vitro

Research output: Contribution to journalJournal articlepeer-review

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Investigating the effect of graphene oxide in chitosan/alginate-based foams on the release and antifungal activity of clotrimazole in vitro. / Huang, J.; Jacobsen, J.; Genina, N.; Larsen, S. W.; Nielsen, H. M.; Müllertz, A.; Mu, H.

In: European Journal of Pharmaceutical Sciences, Vol. 174, 106204, 2022.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Huang, J, Jacobsen, J, Genina, N, Larsen, SW, Nielsen, HM, Müllertz, A & Mu, H 2022, 'Investigating the effect of graphene oxide in chitosan/alginate-based foams on the release and antifungal activity of clotrimazole in vitro', European Journal of Pharmaceutical Sciences, vol. 174, 106204. https://doi.org/10.1016/j.ejps.2022.106204

APA

Huang, J., Jacobsen, J., Genina, N., Larsen, S. W., Nielsen, H. M., Müllertz, A., & Mu, H. (2022). Investigating the effect of graphene oxide in chitosan/alginate-based foams on the release and antifungal activity of clotrimazole in vitro. European Journal of Pharmaceutical Sciences, 174, [106204]. https://doi.org/10.1016/j.ejps.2022.106204

Vancouver

Huang J, Jacobsen J, Genina N, Larsen SW, Nielsen HM, Müllertz A et al. Investigating the effect of graphene oxide in chitosan/alginate-based foams on the release and antifungal activity of clotrimazole in vitro. European Journal of Pharmaceutical Sciences. 2022;174. 106204. https://doi.org/10.1016/j.ejps.2022.106204

Author

Huang, J. ; Jacobsen, J. ; Genina, N. ; Larsen, S. W. ; Nielsen, H. M. ; Müllertz, A. ; Mu, H. / Investigating the effect of graphene oxide in chitosan/alginate-based foams on the release and antifungal activity of clotrimazole in vitro. In: European Journal of Pharmaceutical Sciences. 2022 ; Vol. 174.

Bibtex

@article{12dab079745f4641a38a2b55441bbd40,
title = "Investigating the effect of graphene oxide in chitosan/alginate-based foams on the release and antifungal activity of clotrimazole in vitro",
abstract = "Polyelectrolyte complexes (PECs) have been used as the matrix of solid foams for drug delivery. This study aimed at investigating the effect of graphene oxide (GO) and the composition of excipients in chitosan/alginate-based buccal foams on the clotrimazole release and antifungal activities. The investigation has been focused on the interactions of the drug with excipients in the foams, and the changes of ionization degree upon exposure to various media are discussed. The solid foams were prepared by mixing the excipients and clotrimazole via probe sonication, followed by a freeze-drying method. The pH values of the formulations were measured during the foam preparation process to estimate the ionization degree of clotrimazole and the other excipients. The foam matrix was the PECs between the cationic chitosan and anionic alginate. The mechanical strength of clotrimazole-loaded foams was lower than that of drug-free foams due to the positively charged clotrimazole interacting with the anionic alginate and interfering the PECs between chitosan and alginate. Addition of GO in the clotrimazole-loaded matrix made the foams mechanically stronger and contributed to a faster release of clotrimazole from the buccal foams by disrupting the electrostatic interactions between alginate and clotrimazole. However, addition of 1 wt% GO in the formulations didn't affect the antifungal activity of clotrimazole-loaded foams significantly. A lower amount GO in the formulation may be required for enhancing the antifungal effect, which should be further investigated in future.",
keywords = "Alginate, Antifungal activity, Chitosan, Clotrimazole, Drug release, Polyelectrolyte foam",
author = "J. Huang and J. Jacobsen and N. Genina and Larsen, {S. W.} and Nielsen, {H. M.} and A. M{\"u}llertz and H. Mu",
note = "Funding Information: The financial support by the University of Copenhagen, Denmark and Oversea Study Program of Guangzhou Elite Project for Jianfei Huang (JY201622) is appreciated. We would also like to thank S{\o}ren Michael Nielsen and Dr. Sylvia Natalie K{\l}odzi{\'n}ska for their technical assistance. Funding Information: The financial support by the University of Copenhagen , Denmark and Oversea Study Program of Guangzhou Elite Project for Jianfei Huang ( JY201622 ) is appreciated. We would also like to thank S{\o}ren Michael Nielsen and Dr. Sylvia Natalie K{\l}odzi{\'n}ska for their technical assistance. ",
year = "2022",
doi = "10.1016/j.ejps.2022.106204",
language = "English",
volume = "174",
journal = "Norvegica Pharmaceutica Acta",
issn = "0928-0987",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Investigating the effect of graphene oxide in chitosan/alginate-based foams on the release and antifungal activity of clotrimazole in vitro

AU - Huang, J.

AU - Jacobsen, J.

AU - Genina, N.

AU - Larsen, S. W.

AU - Nielsen, H. M.

AU - Müllertz, A.

AU - Mu, H.

N1 - Funding Information: The financial support by the University of Copenhagen, Denmark and Oversea Study Program of Guangzhou Elite Project for Jianfei Huang (JY201622) is appreciated. We would also like to thank Søren Michael Nielsen and Dr. Sylvia Natalie Kłodzińska for their technical assistance. Funding Information: The financial support by the University of Copenhagen , Denmark and Oversea Study Program of Guangzhou Elite Project for Jianfei Huang ( JY201622 ) is appreciated. We would also like to thank Søren Michael Nielsen and Dr. Sylvia Natalie Kłodzińska for their technical assistance.

PY - 2022

Y1 - 2022

N2 - Polyelectrolyte complexes (PECs) have been used as the matrix of solid foams for drug delivery. This study aimed at investigating the effect of graphene oxide (GO) and the composition of excipients in chitosan/alginate-based buccal foams on the clotrimazole release and antifungal activities. The investigation has been focused on the interactions of the drug with excipients in the foams, and the changes of ionization degree upon exposure to various media are discussed. The solid foams were prepared by mixing the excipients and clotrimazole via probe sonication, followed by a freeze-drying method. The pH values of the formulations were measured during the foam preparation process to estimate the ionization degree of clotrimazole and the other excipients. The foam matrix was the PECs between the cationic chitosan and anionic alginate. The mechanical strength of clotrimazole-loaded foams was lower than that of drug-free foams due to the positively charged clotrimazole interacting with the anionic alginate and interfering the PECs between chitosan and alginate. Addition of GO in the clotrimazole-loaded matrix made the foams mechanically stronger and contributed to a faster release of clotrimazole from the buccal foams by disrupting the electrostatic interactions between alginate and clotrimazole. However, addition of 1 wt% GO in the formulations didn't affect the antifungal activity of clotrimazole-loaded foams significantly. A lower amount GO in the formulation may be required for enhancing the antifungal effect, which should be further investigated in future.

AB - Polyelectrolyte complexes (PECs) have been used as the matrix of solid foams for drug delivery. This study aimed at investigating the effect of graphene oxide (GO) and the composition of excipients in chitosan/alginate-based buccal foams on the clotrimazole release and antifungal activities. The investigation has been focused on the interactions of the drug with excipients in the foams, and the changes of ionization degree upon exposure to various media are discussed. The solid foams were prepared by mixing the excipients and clotrimazole via probe sonication, followed by a freeze-drying method. The pH values of the formulations were measured during the foam preparation process to estimate the ionization degree of clotrimazole and the other excipients. The foam matrix was the PECs between the cationic chitosan and anionic alginate. The mechanical strength of clotrimazole-loaded foams was lower than that of drug-free foams due to the positively charged clotrimazole interacting with the anionic alginate and interfering the PECs between chitosan and alginate. Addition of GO in the clotrimazole-loaded matrix made the foams mechanically stronger and contributed to a faster release of clotrimazole from the buccal foams by disrupting the electrostatic interactions between alginate and clotrimazole. However, addition of 1 wt% GO in the formulations didn't affect the antifungal activity of clotrimazole-loaded foams significantly. A lower amount GO in the formulation may be required for enhancing the antifungal effect, which should be further investigated in future.

KW - Alginate

KW - Antifungal activity

KW - Chitosan

KW - Clotrimazole

KW - Drug release

KW - Polyelectrolyte foam

U2 - 10.1016/j.ejps.2022.106204

DO - 10.1016/j.ejps.2022.106204

M3 - Journal article

C2 - 35550171

AN - SCOPUS:85129966529

VL - 174

JO - Norvegica Pharmaceutica Acta

JF - Norvegica Pharmaceutica Acta

SN - 0928-0987

M1 - 106204

ER -

ID: 308042060