Interactions of Cell-Penetrating Peptide-Modified Nanoparticles with Cells Evaluated Using Single Particle Tracking

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Interactions of Cell-Penetrating Peptide-Modified Nanoparticles with Cells Evaluated Using Single Particle Tracking. / Streck, Sarah; Bohr, Søren S-R; Birch, Ditlev; Rades, Thomas; Hatzakis, Nikos S.; McDowell, Arlene; Nielsen, Hanne Morck.

In: ACS Applied Bio Materials, Vol. 4, No. 4, 2021, p. 3155-3165.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Streck, S, Bohr, SS-R, Birch, D, Rades, T, Hatzakis, NS, McDowell, A & Nielsen, HM 2021, 'Interactions of Cell-Penetrating Peptide-Modified Nanoparticles with Cells Evaluated Using Single Particle Tracking', ACS Applied Bio Materials, vol. 4, no. 4, pp. 3155-3165. https://doi.org/10.1021/acsabm.0c01563

APA

Streck, S., Bohr, S. S-R., Birch, D., Rades, T., Hatzakis, N. S., McDowell, A., & Nielsen, H. M. (2021). Interactions of Cell-Penetrating Peptide-Modified Nanoparticles with Cells Evaluated Using Single Particle Tracking. ACS Applied Bio Materials, 4(4), 3155-3165. https://doi.org/10.1021/acsabm.0c01563

Vancouver

Streck S, Bohr SS-R, Birch D, Rades T, Hatzakis NS, McDowell A et al. Interactions of Cell-Penetrating Peptide-Modified Nanoparticles with Cells Evaluated Using Single Particle Tracking. ACS Applied Bio Materials. 2021;4(4):3155-3165. https://doi.org/10.1021/acsabm.0c01563

Author

Streck, Sarah ; Bohr, Søren S-R ; Birch, Ditlev ; Rades, Thomas ; Hatzakis, Nikos S. ; McDowell, Arlene ; Nielsen, Hanne Morck. / Interactions of Cell-Penetrating Peptide-Modified Nanoparticles with Cells Evaluated Using Single Particle Tracking. In: ACS Applied Bio Materials. 2021 ; Vol. 4, No. 4. pp. 3155-3165.

Bibtex

@article{f2fe18bb984f408498050c4338992002,
title = "Interactions of Cell-Penetrating Peptide-Modified Nanoparticles with Cells Evaluated Using Single Particle Tracking",
abstract = "Cell-penetrating peptides (CPPs) are known to interact with cell membranes and by doing so enhance cellular interaction and subsequent cellular internalization of nanoparticles. Yet, the early events of membrane interactions are still not elucidated, which is the aim of the present work. Surface conjugation of polymeric nanoparticles with cationic CPPs of different architecture (short, long linear, and branched) influences the surface properties, especially the charge of the nanoparticles, and therefore provides the possibility of increased electrostatic interactions between nanoparticles with the cell membrane. In this study, the physicochemical properties of CPP-tagged poly(lactic-co-glycolic acid) (PLGA) nanoparticles were characterized, and nanoparticle-cell interactions were investigated in HeLa cells. With the commonly applied methods of flow cytometry as well as confocal laser scanning microscopy, low and similar levels of nanoparticle association were detected for the PLGA and CPP-tagged PLGA nanoparticles with the cell membrane. However, single particle tracking of CPP-tagged PLGA nanoparticles allowed direct observation of the interactions of individual nanoparticles with cells and consequently elucidated the impact that the CPP architecture on the nanoparticle surface can have. Interestingly, the results revealed that nanoparticles with the branched CPP architecture on the surface displayed decreased diffusion modes likely due to increased interactions with the cell membrane when compared to the other nanoparticles investigated. It is anticipated that single particle approaches like the one used here can be widely employed to reveal currently unresolved characteristics of nanoparticle-cell interaction and aid in the design of improved surface-modified nanoparticles for efficient delivery of therapeutics.",
keywords = "cell-penetrating peptides, branched cell-penetrating peptides, HeLa cells, PLGA, nanoparticles, single particle tracking, PLGA NANOPARTICLES, DELIVERY, DIMERIZATION, DENDRIMERS",
author = "Sarah Streck and Bohr, {S{\o}ren S-R} and Ditlev Birch and Thomas Rades and Hatzakis, {Nikos S.} and Arlene McDowell and Nielsen, {Hanne Morck}",
year = "2021",
doi = "10.1021/acsabm.0c01563",
language = "English",
volume = "4",
pages = "3155--3165",
journal = "ACS Applied Bio Materials",
issn = "2576-6422",
publisher = "American Chemical Society",
number = "4",

}

RIS

TY - JOUR

T1 - Interactions of Cell-Penetrating Peptide-Modified Nanoparticles with Cells Evaluated Using Single Particle Tracking

AU - Streck, Sarah

AU - Bohr, Søren S-R

AU - Birch, Ditlev

AU - Rades, Thomas

AU - Hatzakis, Nikos S.

AU - McDowell, Arlene

AU - Nielsen, Hanne Morck

PY - 2021

Y1 - 2021

N2 - Cell-penetrating peptides (CPPs) are known to interact with cell membranes and by doing so enhance cellular interaction and subsequent cellular internalization of nanoparticles. Yet, the early events of membrane interactions are still not elucidated, which is the aim of the present work. Surface conjugation of polymeric nanoparticles with cationic CPPs of different architecture (short, long linear, and branched) influences the surface properties, especially the charge of the nanoparticles, and therefore provides the possibility of increased electrostatic interactions between nanoparticles with the cell membrane. In this study, the physicochemical properties of CPP-tagged poly(lactic-co-glycolic acid) (PLGA) nanoparticles were characterized, and nanoparticle-cell interactions were investigated in HeLa cells. With the commonly applied methods of flow cytometry as well as confocal laser scanning microscopy, low and similar levels of nanoparticle association were detected for the PLGA and CPP-tagged PLGA nanoparticles with the cell membrane. However, single particle tracking of CPP-tagged PLGA nanoparticles allowed direct observation of the interactions of individual nanoparticles with cells and consequently elucidated the impact that the CPP architecture on the nanoparticle surface can have. Interestingly, the results revealed that nanoparticles with the branched CPP architecture on the surface displayed decreased diffusion modes likely due to increased interactions with the cell membrane when compared to the other nanoparticles investigated. It is anticipated that single particle approaches like the one used here can be widely employed to reveal currently unresolved characteristics of nanoparticle-cell interaction and aid in the design of improved surface-modified nanoparticles for efficient delivery of therapeutics.

AB - Cell-penetrating peptides (CPPs) are known to interact with cell membranes and by doing so enhance cellular interaction and subsequent cellular internalization of nanoparticles. Yet, the early events of membrane interactions are still not elucidated, which is the aim of the present work. Surface conjugation of polymeric nanoparticles with cationic CPPs of different architecture (short, long linear, and branched) influences the surface properties, especially the charge of the nanoparticles, and therefore provides the possibility of increased electrostatic interactions between nanoparticles with the cell membrane. In this study, the physicochemical properties of CPP-tagged poly(lactic-co-glycolic acid) (PLGA) nanoparticles were characterized, and nanoparticle-cell interactions were investigated in HeLa cells. With the commonly applied methods of flow cytometry as well as confocal laser scanning microscopy, low and similar levels of nanoparticle association were detected for the PLGA and CPP-tagged PLGA nanoparticles with the cell membrane. However, single particle tracking of CPP-tagged PLGA nanoparticles allowed direct observation of the interactions of individual nanoparticles with cells and consequently elucidated the impact that the CPP architecture on the nanoparticle surface can have. Interestingly, the results revealed that nanoparticles with the branched CPP architecture on the surface displayed decreased diffusion modes likely due to increased interactions with the cell membrane when compared to the other nanoparticles investigated. It is anticipated that single particle approaches like the one used here can be widely employed to reveal currently unresolved characteristics of nanoparticle-cell interaction and aid in the design of improved surface-modified nanoparticles for efficient delivery of therapeutics.

KW - cell-penetrating peptides

KW - branched cell-penetrating peptides

KW - HeLa cells

KW - PLGA

KW - nanoparticles

KW - single particle tracking

KW - PLGA NANOPARTICLES

KW - DELIVERY

KW - DIMERIZATION

KW - DENDRIMERS

U2 - 10.1021/acsabm.0c01563

DO - 10.1021/acsabm.0c01563

M3 - Journal article

C2 - 35014403

VL - 4

SP - 3155

EP - 3165

JO - ACS Applied Bio Materials

JF - ACS Applied Bio Materials

SN - 2576-6422

IS - 4

ER -

ID: 261211200