In silico design and 3D printing of microfluidic chips for the preparation of size-controllable siRNA nanocomplexes

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

In silico design and 3D printing of microfluidic chips for the preparation of size-controllable siRNA nanocomplexes. / Li, Yongquan; Bøtker, Johan; Rantanen, Jukka; Yang, Mingshi; Bohr, Adam.

In: International Journal of Pharmaceutics, Vol. 583, 119388, 15.06.2020.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Li, Y, Bøtker, J, Rantanen, J, Yang, M & Bohr, A 2020, 'In silico design and 3D printing of microfluidic chips for the preparation of size-controllable siRNA nanocomplexes', International Journal of Pharmaceutics, vol. 583, 119388. https://doi.org/10.1016/j.ijpharm.2020.119388

APA

Li, Y., Bøtker, J., Rantanen, J., Yang, M., & Bohr, A. (2020). In silico design and 3D printing of microfluidic chips for the preparation of size-controllable siRNA nanocomplexes. International Journal of Pharmaceutics, 583, [119388]. https://doi.org/10.1016/j.ijpharm.2020.119388

Vancouver

Li Y, Bøtker J, Rantanen J, Yang M, Bohr A. In silico design and 3D printing of microfluidic chips for the preparation of size-controllable siRNA nanocomplexes. International Journal of Pharmaceutics. 2020 Jun 15;583. 119388. https://doi.org/10.1016/j.ijpharm.2020.119388

Author

Li, Yongquan ; Bøtker, Johan ; Rantanen, Jukka ; Yang, Mingshi ; Bohr, Adam. / In silico design and 3D printing of microfluidic chips for the preparation of size-controllable siRNA nanocomplexes. In: International Journal of Pharmaceutics. 2020 ; Vol. 583.

Bibtex

@article{96f2af451ba645409148012e36a7545e,
title = "In silico design and 3D printing of microfluidic chips for the preparation of size-controllable siRNA nanocomplexes",
abstract = "Small interfering RNA (siRNA) is regarded as one of the most powerful tools for the treatment of various diseases by downregulating the expression of aberrant proteins. Delivery vehicle is often necessary for getting siRNA into the cells. Nanocomplex using polyamidoamine (PAMAM) is regarded a promising approach for the delivery of siRNA. The size of siRNA nanocomplexes is a critical attribute in order to achieve high gene silencing efficiency in vivo. Microfluidics provides advantages in the preparation of siRNA nanocomplexes due to better reproducibility and a potential for more robust process control. The mixing efficiency of siRNA and PAMAM is different in microfluidics systems with different geometries, therefore, resulting in nanocomplexes with varying size attributes. In this study, hydrodynamic flow focusing microfluidic chips with different channel designs, i.e. diameters/widths, channel shapes (cylindrical/rectangular) and inter-channel spacings were optimized in silico and rapidly prototyped using 3D printing and finally, used for production of siRNA nanocomplexes. The fluid mixing inside the microfluidic chips was simulated using the finite element method (FEM) with the single-phase laminar flow interface in connection with the transport of diluted species interface. The digital design and optimization of microfluidic chips showed consistency with experimental results. It was concluded that the size of siRNA nanocomplexes can be controlled by adjusting the channel geometry of the microfluidic chips and the simulation with FEM could be used to facilitate the design and optimization of microfluidic chips in order to produce nanocomplexes with desirable attributes.",
author = "Yongquan Li and Johan B{\o}tker and Jukka Rantanen and Mingshi Yang and Adam Bohr",
note = "Copyright {\circledC} 2020 Elsevier B.V. All rights reserved.",
year = "2020",
month = "6",
day = "15",
doi = "10.1016/j.ijpharm.2020.119388",
language = "English",
volume = "583",
journal = "International Journal of Pharmaceutics",
issn = "0378-5173",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - In silico design and 3D printing of microfluidic chips for the preparation of size-controllable siRNA nanocomplexes

AU - Li, Yongquan

AU - Bøtker, Johan

AU - Rantanen, Jukka

AU - Yang, Mingshi

AU - Bohr, Adam

N1 - Copyright © 2020 Elsevier B.V. All rights reserved.

PY - 2020/6/15

Y1 - 2020/6/15

N2 - Small interfering RNA (siRNA) is regarded as one of the most powerful tools for the treatment of various diseases by downregulating the expression of aberrant proteins. Delivery vehicle is often necessary for getting siRNA into the cells. Nanocomplex using polyamidoamine (PAMAM) is regarded a promising approach for the delivery of siRNA. The size of siRNA nanocomplexes is a critical attribute in order to achieve high gene silencing efficiency in vivo. Microfluidics provides advantages in the preparation of siRNA nanocomplexes due to better reproducibility and a potential for more robust process control. The mixing efficiency of siRNA and PAMAM is different in microfluidics systems with different geometries, therefore, resulting in nanocomplexes with varying size attributes. In this study, hydrodynamic flow focusing microfluidic chips with different channel designs, i.e. diameters/widths, channel shapes (cylindrical/rectangular) and inter-channel spacings were optimized in silico and rapidly prototyped using 3D printing and finally, used for production of siRNA nanocomplexes. The fluid mixing inside the microfluidic chips was simulated using the finite element method (FEM) with the single-phase laminar flow interface in connection with the transport of diluted species interface. The digital design and optimization of microfluidic chips showed consistency with experimental results. It was concluded that the size of siRNA nanocomplexes can be controlled by adjusting the channel geometry of the microfluidic chips and the simulation with FEM could be used to facilitate the design and optimization of microfluidic chips in order to produce nanocomplexes with desirable attributes.

AB - Small interfering RNA (siRNA) is regarded as one of the most powerful tools for the treatment of various diseases by downregulating the expression of aberrant proteins. Delivery vehicle is often necessary for getting siRNA into the cells. Nanocomplex using polyamidoamine (PAMAM) is regarded a promising approach for the delivery of siRNA. The size of siRNA nanocomplexes is a critical attribute in order to achieve high gene silencing efficiency in vivo. Microfluidics provides advantages in the preparation of siRNA nanocomplexes due to better reproducibility and a potential for more robust process control. The mixing efficiency of siRNA and PAMAM is different in microfluidics systems with different geometries, therefore, resulting in nanocomplexes with varying size attributes. In this study, hydrodynamic flow focusing microfluidic chips with different channel designs, i.e. diameters/widths, channel shapes (cylindrical/rectangular) and inter-channel spacings were optimized in silico and rapidly prototyped using 3D printing and finally, used for production of siRNA nanocomplexes. The fluid mixing inside the microfluidic chips was simulated using the finite element method (FEM) with the single-phase laminar flow interface in connection with the transport of diluted species interface. The digital design and optimization of microfluidic chips showed consistency with experimental results. It was concluded that the size of siRNA nanocomplexes can be controlled by adjusting the channel geometry of the microfluidic chips and the simulation with FEM could be used to facilitate the design and optimization of microfluidic chips in order to produce nanocomplexes with desirable attributes.

U2 - 10.1016/j.ijpharm.2020.119388

DO - 10.1016/j.ijpharm.2020.119388

M3 - Journal article

C2 - 32376446

VL - 583

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

M1 - 119388

ER -

ID: 242473201