HPLC method validated for the simultaneous analysis of cichoric acid and alkamides in Echinacea purpurea plants and products
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HPLC method validated for the simultaneous analysis of cichoric acid and alkamides in Echinacea purpurea plants and products. / Mølgaard, Per; Johnsen, Søren; Christensen, Peter; Cornett, Claus.
In: Journal of Agricultural and Food Chemistry, Vol. 51, No. 24, 2003, p. 6922-33.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - HPLC method validated for the simultaneous analysis of cichoric acid and alkamides in Echinacea purpurea plants and products
AU - Mølgaard, Per
AU - Johnsen, Søren
AU - Christensen, Peter
AU - Cornett, Claus
N1 - Keywords: Amides; Caffeic Acids; Chromatography, High Pressure Liquid; Denmark; Echinacea; Plant Extracts; Plant Preparations; Quality Control; Succinates
PY - 2003
Y1 - 2003
N2 - A reversed-phase high-performance liquid chromatography (HPLC) method has been developed to determine caffeic acid derivatives, for example, cichoric acid, and alkamides in plant parts and herbal products of Echinacea purpurea. The method consists of an extraction procedure whereby the hydrophilic phenolics as well as the lipophilic alkamides are released from the samples, followed by the analytical HPLC procedure for quantitative determination of these compounds. The method is the first one validated for the determination of these two groups of compounds in the same procedure. Naringenin has been used as an internal standard, as no other flavanones are present in the extract and it does not interfere with any of the compounds under investigation. Analysis of Danish-grown plant material shows that it is possible to raise plants of a very high chemical quality in Denmark. A selection of international herbal products available on the Danish market show surprisingly variable quality, not necessarily reflecting the product information given on the labels.
AB - A reversed-phase high-performance liquid chromatography (HPLC) method has been developed to determine caffeic acid derivatives, for example, cichoric acid, and alkamides in plant parts and herbal products of Echinacea purpurea. The method consists of an extraction procedure whereby the hydrophilic phenolics as well as the lipophilic alkamides are released from the samples, followed by the analytical HPLC procedure for quantitative determination of these compounds. The method is the first one validated for the determination of these two groups of compounds in the same procedure. Naringenin has been used as an internal standard, as no other flavanones are present in the extract and it does not interfere with any of the compounds under investigation. Analysis of Danish-grown plant material shows that it is possible to raise plants of a very high chemical quality in Denmark. A selection of international herbal products available on the Danish market show surprisingly variable quality, not necessarily reflecting the product information given on the labels.
U2 - 10.1021/jf026158f
DO - 10.1021/jf026158f
M3 - Journal article
C2 - 14611148
VL - 51
SP - 6922
EP - 6933
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 24
ER -
ID: 9040256