High-Throughput Lipolysis in 96-Well Plates for Rapid Screening of Lipid-Based Drug Delivery Systems

Research output: Contribution to journalJournal article

Standard

High-Throughput Lipolysis in 96-Well Plates for Rapid Screening of Lipid-Based Drug Delivery Systems. / Mosgaard, Mette D; Sassene, Philip J; Mu, Huiling; Rades, Thomas; Müllertz, Anette.

In: Journal of Pharmaceutical Sciences, Vol. 106, No. 4, 04.2017, p. 1183-1186.

Research output: Contribution to journalJournal article

Harvard

Mosgaard, MD, Sassene, PJ, Mu, H, Rades, T & Müllertz, A 2017, 'High-Throughput Lipolysis in 96-Well Plates for Rapid Screening of Lipid-Based Drug Delivery Systems', Journal of Pharmaceutical Sciences, vol. 106, no. 4, pp. 1183-1186. https://doi.org/10.1016/j.xphs.2016.12.026

APA

Mosgaard, M. D., Sassene, P. J., Mu, H., Rades, T., & Müllertz, A. (2017). High-Throughput Lipolysis in 96-Well Plates for Rapid Screening of Lipid-Based Drug Delivery Systems. Journal of Pharmaceutical Sciences, 106(4), 1183-1186. https://doi.org/10.1016/j.xphs.2016.12.026

Vancouver

Mosgaard MD, Sassene PJ, Mu H, Rades T, Müllertz A. High-Throughput Lipolysis in 96-Well Plates for Rapid Screening of Lipid-Based Drug Delivery Systems. Journal of Pharmaceutical Sciences. 2017 Apr;106(4):1183-1186. https://doi.org/10.1016/j.xphs.2016.12.026

Author

Mosgaard, Mette D ; Sassene, Philip J ; Mu, Huiling ; Rades, Thomas ; Müllertz, Anette. / High-Throughput Lipolysis in 96-Well Plates for Rapid Screening of Lipid-Based Drug Delivery Systems. In: Journal of Pharmaceutical Sciences. 2017 ; Vol. 106, No. 4. pp. 1183-1186.

Bibtex

@article{cc5c1fdec3624bfb8353b2ed9d9e62b9,
title = "High-Throughput Lipolysis in 96-Well Plates for Rapid Screening of Lipid-Based Drug Delivery Systems",
abstract = "The high-throughput in vitro intestinal lipolysis model (HTP) applicable for rapid and low-scale screening of lipid-based drug delivery systems (LbDDSs) was optimized and adjusted as to be conducted in 96-well plates (HTP-96). Three different LbDDSs (I-III) loaded with danazol or cinnarizine were used as model systems. The distributions of cinnarizine and danazol in the aqueous and precipitated digestion phases generated during lipolysis in HTP-96 were compared with previously published data obtained from HTP. The final HTP-96 setup resulted in the same rank order as the original HTP model with regard to solubilization in the aqueous phase during digestion: LbDDS III > LbDDS II > LbDDS I for danazol and LbDDS III ≈ LbDDS II ≈ LbDDS I for cinnarizine. HTP-96 is a useful model for fast performance assessment of LbDDS in a small scale.",
keywords = "Journal Article",
author = "Mosgaard, {Mette D} and Sassene, {Philip J} and Huiling Mu and Thomas Rades and Anette M{\"u}llertz",
note = "Copyright {\circledC} 2017 American Pharmacists Association{\circledR}. Published by Elsevier Inc. All rights reserved.",
year = "2017",
month = "4",
doi = "10.1016/j.xphs.2016.12.026",
language = "English",
volume = "106",
pages = "1183--1186",
journal = "Journal of Pharmaceutical Sciences",
issn = "0022-3549",
publisher = "Elsevier",
number = "4",

}

RIS

TY - JOUR

T1 - High-Throughput Lipolysis in 96-Well Plates for Rapid Screening of Lipid-Based Drug Delivery Systems

AU - Mosgaard, Mette D

AU - Sassene, Philip J

AU - Mu, Huiling

AU - Rades, Thomas

AU - Müllertz, Anette

N1 - Copyright © 2017 American Pharmacists Association®. Published by Elsevier Inc. All rights reserved.

PY - 2017/4

Y1 - 2017/4

N2 - The high-throughput in vitro intestinal lipolysis model (HTP) applicable for rapid and low-scale screening of lipid-based drug delivery systems (LbDDSs) was optimized and adjusted as to be conducted in 96-well plates (HTP-96). Three different LbDDSs (I-III) loaded with danazol or cinnarizine were used as model systems. The distributions of cinnarizine and danazol in the aqueous and precipitated digestion phases generated during lipolysis in HTP-96 were compared with previously published data obtained from HTP. The final HTP-96 setup resulted in the same rank order as the original HTP model with regard to solubilization in the aqueous phase during digestion: LbDDS III > LbDDS II > LbDDS I for danazol and LbDDS III ≈ LbDDS II ≈ LbDDS I for cinnarizine. HTP-96 is a useful model for fast performance assessment of LbDDS in a small scale.

AB - The high-throughput in vitro intestinal lipolysis model (HTP) applicable for rapid and low-scale screening of lipid-based drug delivery systems (LbDDSs) was optimized and adjusted as to be conducted in 96-well plates (HTP-96). Three different LbDDSs (I-III) loaded with danazol or cinnarizine were used as model systems. The distributions of cinnarizine and danazol in the aqueous and precipitated digestion phases generated during lipolysis in HTP-96 were compared with previously published data obtained from HTP. The final HTP-96 setup resulted in the same rank order as the original HTP model with regard to solubilization in the aqueous phase during digestion: LbDDS III > LbDDS II > LbDDS I for danazol and LbDDS III ≈ LbDDS II ≈ LbDDS I for cinnarizine. HTP-96 is a useful model for fast performance assessment of LbDDS in a small scale.

KW - Journal Article

U2 - 10.1016/j.xphs.2016.12.026

DO - 10.1016/j.xphs.2016.12.026

M3 - Journal article

C2 - 28057543

VL - 106

SP - 1183

EP - 1186

JO - Journal of Pharmaceutical Sciences

JF - Journal of Pharmaceutical Sciences

SN - 0022-3549

IS - 4

ER -

ID: 185745485