High-performance liquid chromatography on-line coupled to high-field NMR and mass spectrometry for structure elucidation of constituents of Hypericum perforatum L
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High-performance liquid chromatography on-line coupled to high-field NMR and mass spectrometry for structure elucidation of constituents of Hypericum perforatum L. / Hansen, S. H.; Jensen, A. G.; Cornett, Claus; Bjornsdottir, I.; Taylor, S.; Wright, B.; Wilson, I. D.
In: Analytical Chemistry, Vol. 71, No. 22, 1999, p. 5235-5241.Research output: Contribution to journal › Journal article › peer-review
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T1 - High-performance liquid chromatography on-line coupled to high-field NMR and mass spectrometry for structure elucidation of constituents of Hypericum perforatum L
AU - Hansen, S. H.
AU - Jensen, A. G.
AU - Cornett, Claus
AU - Bjornsdottir, I.
AU - Taylor, S.
AU - Wright, B.
AU - Wilson, I. D.
PY - 1999
Y1 - 1999
N2 - The on-line separation and structure elucidation of naphthodianthrones, flavonoids, and other constituents of an extract from Hypericum perforatum L, using high performance liquid chromatography (HPLC) coupled on-line with ultraviolet-visible, nuclear magnetic resonance (NMR), and mass spectrometry (MS) is described. A conventional reversed-phase HPLC system using ammonium acetate as the buffer substance in the eluent tvas used, and proton NMR spectra were obtained on a 500 MHz NMR instrument. The MS and MS/MS analyses were performed using negative electrospray ionization, In the present study, all of the major known constituents in extracts from Hypericum perforatum L, were identified, and two new substances which had not previously been reported as constituents of extracts of Hypericum perforatum L. were identified and their structures elucidated.
AB - The on-line separation and structure elucidation of naphthodianthrones, flavonoids, and other constituents of an extract from Hypericum perforatum L, using high performance liquid chromatography (HPLC) coupled on-line with ultraviolet-visible, nuclear magnetic resonance (NMR), and mass spectrometry (MS) is described. A conventional reversed-phase HPLC system using ammonium acetate as the buffer substance in the eluent tvas used, and proton NMR spectra were obtained on a 500 MHz NMR instrument. The MS and MS/MS analyses were performed using negative electrospray ionization, In the present study, all of the major known constituents in extracts from Hypericum perforatum L, were identified, and two new substances which had not previously been reported as constituents of extracts of Hypericum perforatum L. were identified and their structures elucidated.
KW - identification products
M3 - Tidsskriftartikel
VL - 71
SP - 5235
EP - 5241
JO - Industrial And Engineering Chemistry Analytical Edition
JF - Industrial And Engineering Chemistry Analytical Edition
SN - 0003-2700
IS - 22
ER -
ID: 38061363