Evaluation of statistical design/modeling for prediction of the effect of amino acids on agitation-induced aggregation of human growth hormone and human insulin

Research output: Contribution to journalJournal articlepeer-review

Standard

Evaluation of statistical design/modeling for prediction of the effect of amino acids on agitation-induced aggregation of human growth hormone and human insulin. / Soenderkaer, S.; Van De Weert, M.; Lindgaard Hansen, L.; Flink, J.; Frokjaer, S.

In: Journal of Drug Delivery Science and Technology, Vol. 15, No. 6, 2005, p. 427-434.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Soenderkaer, S, Van De Weert, M, Lindgaard Hansen, L, Flink, J & Frokjaer, S 2005, 'Evaluation of statistical design/modeling for prediction of the effect of amino acids on agitation-induced aggregation of human growth hormone and human insulin', Journal of Drug Delivery Science and Technology, vol. 15, no. 6, pp. 427-434. https://doi.org/10.1016/s1773-2247(05)50083-2

APA

Soenderkaer, S., Van De Weert, M., Lindgaard Hansen, L., Flink, J., & Frokjaer, S. (2005). Evaluation of statistical design/modeling for prediction of the effect of amino acids on agitation-induced aggregation of human growth hormone and human insulin. Journal of Drug Delivery Science and Technology, 15(6), 427-434. https://doi.org/10.1016/s1773-2247(05)50083-2

Vancouver

Soenderkaer S, Van De Weert M, Lindgaard Hansen L, Flink J, Frokjaer S. Evaluation of statistical design/modeling for prediction of the effect of amino acids on agitation-induced aggregation of human growth hormone and human insulin. Journal of Drug Delivery Science and Technology. 2005;15(6):427-434. https://doi.org/10.1016/s1773-2247(05)50083-2

Author

Soenderkaer, S. ; Van De Weert, M. ; Lindgaard Hansen, L. ; Flink, J. ; Frokjaer, S. / Evaluation of statistical design/modeling for prediction of the effect of amino acids on agitation-induced aggregation of human growth hormone and human insulin. In: Journal of Drug Delivery Science and Technology. 2005 ; Vol. 15, No. 6. pp. 427-434.

Bibtex

@article{d46d4e3443e34ca8a0395208c69d921b,
title = "Evaluation of statistical design/modeling for prediction of the effect of amino acids on agitation-induced aggregation of human growth hormone and human insulin",
abstract = "This study investigated how the physical-chemical characteristics of natural amino acids influence the conformational stability of recombinant human growth hormone (rhGH) and hexameric recombinant human insulin. A statistical design, with a set of physical-chemical descriptors, was used to ensure selection of natural amino acids having a wide range of properties. Amino acids were found to affect agitation induced aggregation of rhGH, as well as insulin. Of particular interest, a substantial stabilization of rhGH occurred in the presence of arginine. In contrast, insulin was destabilized in the presence of all the amino acids selected. A model correlating amino acid physicochemical properties and rhGH agitation-induced aggregation was established. However, no such model could be constructed for the insulin stability data. Thus, it remains questionable whether a simple set of physical-chemical descriptors can sufficiently reflect the general impact of amino acids on the delicate balance of forces needed to maintain protein physical stability.",
keywords = "Agitation-induced aggregation, Amino acids, FTIR, Human growth hormone, Human insulin",
author = "S. Soenderkaer and {Van De Weert}, M. and {Lindgaard Hansen}, L. and J. Flink and S. Frokjaer",
year = "2005",
doi = "10.1016/s1773-2247(05)50083-2",
language = "English",
volume = "15",
pages = "427--434",
journal = "Journal of Drug Delivery Science and Technology",
issn = "1773-2247",
publisher = "Elsevier",
number = "6",

}

RIS

TY - JOUR

T1 - Evaluation of statistical design/modeling for prediction of the effect of amino acids on agitation-induced aggregation of human growth hormone and human insulin

AU - Soenderkaer, S.

AU - Van De Weert, M.

AU - Lindgaard Hansen, L.

AU - Flink, J.

AU - Frokjaer, S.

PY - 2005

Y1 - 2005

N2 - This study investigated how the physical-chemical characteristics of natural amino acids influence the conformational stability of recombinant human growth hormone (rhGH) and hexameric recombinant human insulin. A statistical design, with a set of physical-chemical descriptors, was used to ensure selection of natural amino acids having a wide range of properties. Amino acids were found to affect agitation induced aggregation of rhGH, as well as insulin. Of particular interest, a substantial stabilization of rhGH occurred in the presence of arginine. In contrast, insulin was destabilized in the presence of all the amino acids selected. A model correlating amino acid physicochemical properties and rhGH agitation-induced aggregation was established. However, no such model could be constructed for the insulin stability data. Thus, it remains questionable whether a simple set of physical-chemical descriptors can sufficiently reflect the general impact of amino acids on the delicate balance of forces needed to maintain protein physical stability.

AB - This study investigated how the physical-chemical characteristics of natural amino acids influence the conformational stability of recombinant human growth hormone (rhGH) and hexameric recombinant human insulin. A statistical design, with a set of physical-chemical descriptors, was used to ensure selection of natural amino acids having a wide range of properties. Amino acids were found to affect agitation induced aggregation of rhGH, as well as insulin. Of particular interest, a substantial stabilization of rhGH occurred in the presence of arginine. In contrast, insulin was destabilized in the presence of all the amino acids selected. A model correlating amino acid physicochemical properties and rhGH agitation-induced aggregation was established. However, no such model could be constructed for the insulin stability data. Thus, it remains questionable whether a simple set of physical-chemical descriptors can sufficiently reflect the general impact of amino acids on the delicate balance of forces needed to maintain protein physical stability.

KW - Agitation-induced aggregation

KW - Amino acids

KW - FTIR

KW - Human growth hormone

KW - Human insulin

U2 - 10.1016/s1773-2247(05)50083-2

DO - 10.1016/s1773-2247(05)50083-2

M3 - Journal article

AN - SCOPUS:28144453887

VL - 15

SP - 427

EP - 434

JO - Journal of Drug Delivery Science and Technology

JF - Journal of Drug Delivery Science and Technology

SN - 1773-2247

IS - 6

ER -

ID: 198780053