Effects of recombinant human gastric lipase and pancreatin during in vitro pediatric gastro-intestinal digestion

Research output: Contribution to journalJournal articlepeer-review

Standard

Effects of recombinant human gastric lipase and pancreatin during in vitro pediatric gastro-intestinal digestion. / Heerup, Christine; Ebbesen, Morten Frendø; Geng, Xiaolu; Madsen, Sofie Falkenløve; Berthelsen, Ragna; Müllertz, Anette.

In: Food & Function, Vol. 12, No. 7, 2021, p. 2938-2949.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Heerup, C, Ebbesen, MF, Geng, X, Madsen, SF, Berthelsen, R & Müllertz, A 2021, 'Effects of recombinant human gastric lipase and pancreatin during in vitro pediatric gastro-intestinal digestion', Food & Function, vol. 12, no. 7, pp. 2938-2949. https://doi.org/10.1039/d0fo02976a

APA

Heerup, C., Ebbesen, M. F., Geng, X., Madsen, S. F., Berthelsen, R., & Müllertz, A. (2021). Effects of recombinant human gastric lipase and pancreatin during in vitro pediatric gastro-intestinal digestion. Food & Function, 12(7), 2938-2949. https://doi.org/10.1039/d0fo02976a

Vancouver

Heerup C, Ebbesen MF, Geng X, Madsen SF, Berthelsen R, Müllertz A. Effects of recombinant human gastric lipase and pancreatin during in vitro pediatric gastro-intestinal digestion. Food & Function. 2021;12(7):2938-2949. https://doi.org/10.1039/d0fo02976a

Author

Heerup, Christine ; Ebbesen, Morten Frendø ; Geng, Xiaolu ; Madsen, Sofie Falkenløve ; Berthelsen, Ragna ; Müllertz, Anette. / Effects of recombinant human gastric lipase and pancreatin during in vitro pediatric gastro-intestinal digestion. In: Food & Function. 2021 ; Vol. 12, No. 7. pp. 2938-2949.

Bibtex

@article{3be60dc719cc4394ac8887dfd6c49dd1,
title = "Effects of recombinant human gastric lipase and pancreatin during in vitro pediatric gastro-intestinal digestion",
abstract = "The aim of the study was to implement a gastric digestion step using recombinant human gastric lipase (rHGL) in an in vitro pediatric gastro-intestinal digestion model to achieve a physiologically relevant gastric contribution to total gastro-intestinal lipid digestion. A commercial infant formula (NAN Comfort stage 1 (NAN1)) with 3.4% lipid and an in-lab prepared oil-in-water emulsion, emulsified with soy phosphatidylcholine (SPCemul), with 3.5% lipid (oil-blend containing Akonino NS, MEG-3 and ARASCO oils) were subjected to in vitro gastro-intestinal digestion. To achieve a physiologically relevant level of gastric digestion, 50 min of in vitro gastric digestion, using either 0, 3.75 or 7.5 TBU mL-1 rHGL, was followed by 90 min of in vitro intestinal digestion, using either 0 or 26.5 TBU mL-1 pancreatic triglyceride lipase (PTL) from porcine pancreatin. The digestion of the substrates was assessed using titration-based quantification supported by HPLC-ELSD analysis. In vitro gastric digestion of NAN1 and SPCemul with either 3.75 or 7.5 TBU mL-1 rHGL contributed with 10-27% of the total gastro-intestinal digestion, corresponding to the reported contribution in human infants. At the end of the gastro-intestinal digestion (t = 140 min), the combined lipolytic effect of rHGL and PTL was additive during digestion of SPCemul, but not for the digestion of NAN1, as all lipase activity combinations resulted in a similar degree of NAN1 digestion. The effect of gastric digestion with rHGL on total digestion therefore appeared to be substrate dependent. To conclude, a gastric digestion step using rHGL resulting in physiologically relevant gastric contribution to the observed gastro-intestinal digestion was successfully implemented into an in vitro pediatric gastro-intestinal digestion model.",
author = "Christine Heerup and Ebbesen, {Morten Frend{\o}} and Xiaolu Geng and Madsen, {Sofie Falkenl{\o}ve} and Ragna Berthelsen and Anette M{\"u}llertz",
year = "2021",
doi = "10.1039/d0fo02976a",
language = "English",
volume = "12",
pages = "2938--2949",
journal = "Food & Function",
issn = "2042-6496",
publisher = "Royal Society of Chemistry",
number = "7",

}

RIS

TY - JOUR

T1 - Effects of recombinant human gastric lipase and pancreatin during in vitro pediatric gastro-intestinal digestion

AU - Heerup, Christine

AU - Ebbesen, Morten Frendø

AU - Geng, Xiaolu

AU - Madsen, Sofie Falkenløve

AU - Berthelsen, Ragna

AU - Müllertz, Anette

PY - 2021

Y1 - 2021

N2 - The aim of the study was to implement a gastric digestion step using recombinant human gastric lipase (rHGL) in an in vitro pediatric gastro-intestinal digestion model to achieve a physiologically relevant gastric contribution to total gastro-intestinal lipid digestion. A commercial infant formula (NAN Comfort stage 1 (NAN1)) with 3.4% lipid and an in-lab prepared oil-in-water emulsion, emulsified with soy phosphatidylcholine (SPCemul), with 3.5% lipid (oil-blend containing Akonino NS, MEG-3 and ARASCO oils) were subjected to in vitro gastro-intestinal digestion. To achieve a physiologically relevant level of gastric digestion, 50 min of in vitro gastric digestion, using either 0, 3.75 or 7.5 TBU mL-1 rHGL, was followed by 90 min of in vitro intestinal digestion, using either 0 or 26.5 TBU mL-1 pancreatic triglyceride lipase (PTL) from porcine pancreatin. The digestion of the substrates was assessed using titration-based quantification supported by HPLC-ELSD analysis. In vitro gastric digestion of NAN1 and SPCemul with either 3.75 or 7.5 TBU mL-1 rHGL contributed with 10-27% of the total gastro-intestinal digestion, corresponding to the reported contribution in human infants. At the end of the gastro-intestinal digestion (t = 140 min), the combined lipolytic effect of rHGL and PTL was additive during digestion of SPCemul, but not for the digestion of NAN1, as all lipase activity combinations resulted in a similar degree of NAN1 digestion. The effect of gastric digestion with rHGL on total digestion therefore appeared to be substrate dependent. To conclude, a gastric digestion step using rHGL resulting in physiologically relevant gastric contribution to the observed gastro-intestinal digestion was successfully implemented into an in vitro pediatric gastro-intestinal digestion model.

AB - The aim of the study was to implement a gastric digestion step using recombinant human gastric lipase (rHGL) in an in vitro pediatric gastro-intestinal digestion model to achieve a physiologically relevant gastric contribution to total gastro-intestinal lipid digestion. A commercial infant formula (NAN Comfort stage 1 (NAN1)) with 3.4% lipid and an in-lab prepared oil-in-water emulsion, emulsified with soy phosphatidylcholine (SPCemul), with 3.5% lipid (oil-blend containing Akonino NS, MEG-3 and ARASCO oils) were subjected to in vitro gastro-intestinal digestion. To achieve a physiologically relevant level of gastric digestion, 50 min of in vitro gastric digestion, using either 0, 3.75 or 7.5 TBU mL-1 rHGL, was followed by 90 min of in vitro intestinal digestion, using either 0 or 26.5 TBU mL-1 pancreatic triglyceride lipase (PTL) from porcine pancreatin. The digestion of the substrates was assessed using titration-based quantification supported by HPLC-ELSD analysis. In vitro gastric digestion of NAN1 and SPCemul with either 3.75 or 7.5 TBU mL-1 rHGL contributed with 10-27% of the total gastro-intestinal digestion, corresponding to the reported contribution in human infants. At the end of the gastro-intestinal digestion (t = 140 min), the combined lipolytic effect of rHGL and PTL was additive during digestion of SPCemul, but not for the digestion of NAN1, as all lipase activity combinations resulted in a similar degree of NAN1 digestion. The effect of gastric digestion with rHGL on total digestion therefore appeared to be substrate dependent. To conclude, a gastric digestion step using rHGL resulting in physiologically relevant gastric contribution to the observed gastro-intestinal digestion was successfully implemented into an in vitro pediatric gastro-intestinal digestion model.

U2 - 10.1039/d0fo02976a

DO - 10.1039/d0fo02976a

M3 - Journal article

C2 - 33710204

AN - SCOPUS:85099986690

VL - 12

SP - 2938

EP - 2949

JO - Food & Function

JF - Food & Function

SN - 2042-6496

IS - 7

ER -

ID: 261105243