Determination of methylphenidate in Calliphorid larvae by liquid-liquid extraction and liquid chromatography mass spectrometry - Forensic entomotoxicology using an in vivo rat brain model

Research output: Contribution to journalJournal articleResearchpeer-review

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Determination of methylphenidate in Calliphorid larvae by liquid-liquid extraction and liquid chromatography mass spectrometry - Forensic entomotoxicology using an in vivo rat brain model. / Bushby, Sarah K.; Thomas, Nicky; Priemel, Petra A.; Coulter, Carolyn V.; Rades, Thomas; Kieser, Jules A.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 70, 2012, p. 456-61.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Bushby, SK, Thomas, N, Priemel, PA, Coulter, CV, Rades, T & Kieser, JA 2012, 'Determination of methylphenidate in Calliphorid larvae by liquid-liquid extraction and liquid chromatography mass spectrometry - Forensic entomotoxicology using an in vivo rat brain model', Journal of Pharmaceutical and Biomedical Analysis, vol. 70, pp. 456-61. https://doi.org/10.1016/j.jpba.2012.06.024

APA

Bushby, S. K., Thomas, N., Priemel, P. A., Coulter, C. V., Rades, T., & Kieser, J. A. (2012). Determination of methylphenidate in Calliphorid larvae by liquid-liquid extraction and liquid chromatography mass spectrometry - Forensic entomotoxicology using an in vivo rat brain model. Journal of Pharmaceutical and Biomedical Analysis, 70, 456-61. https://doi.org/10.1016/j.jpba.2012.06.024

Vancouver

Bushby SK, Thomas N, Priemel PA, Coulter CV, Rades T, Kieser JA. Determination of methylphenidate in Calliphorid larvae by liquid-liquid extraction and liquid chromatography mass spectrometry - Forensic entomotoxicology using an in vivo rat brain model. Journal of Pharmaceutical and Biomedical Analysis. 2012;70:456-61. https://doi.org/10.1016/j.jpba.2012.06.024

Author

Bushby, Sarah K. ; Thomas, Nicky ; Priemel, Petra A. ; Coulter, Carolyn V. ; Rades, Thomas ; Kieser, Jules A. / Determination of methylphenidate in Calliphorid larvae by liquid-liquid extraction and liquid chromatography mass spectrometry - Forensic entomotoxicology using an in vivo rat brain model. In: Journal of Pharmaceutical and Biomedical Analysis. 2012 ; Vol. 70. pp. 456-61.

Bibtex

@article{c351d91626844283b60874e52ca7b47e,
title = "Determination of methylphenidate in Calliphorid larvae by liquid-liquid extraction and liquid chromatography mass spectrometry - Forensic entomotoxicology using an in vivo rat brain model",
abstract = "The aim of this study was to examine the potential forensic utilisation of blowfly larvae (Diptera: Calliphoridae) as an alternative toxicological specimen for the detection of the psychotropic model drug methylphenidate (MPH). MPH was extracted from biological matrices (rat brain, serum and Calliphorid larvae) by liquid-liquid extraction with recovery of >80{\%}, and quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS assay was validated for entomotoxicological use and initially applied to male Sprague-Dawley rats (n=6) that were dosed with MPH (20mg/kg) ante-mortem. MPH could be detected in Calliphorid larvae (n=15) reared on the rat brains at 3.2±1.6ng/g. Secondly, MPH-spiked porcine brain tissue (450mg/kg) was used to investigate drug concentration in larvae over a period of 72h. After larvae had feed for 60h, MPH was detected at 19.8±1.4µg/g in the feeding larvae and at 3.5±0.1µg/g in the MPH-spiked porcine brain tissue. It could be advantageous to use Calliphorid larvae as an alternative toxicological specimen to detect alkaline labile drugs, such as MPH.",
author = "Bushby, {Sarah K.} and Nicky Thomas and Priemel, {Petra A.} and Coulter, {Carolyn V.} and Thomas Rades and Kieser, {Jules A.}",
note = "Copyright {\circledC} 2012 Elsevier B.V. All rights reserved.",
year = "2012",
doi = "10.1016/j.jpba.2012.06.024",
language = "English",
volume = "70",
pages = "456--61",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Determination of methylphenidate in Calliphorid larvae by liquid-liquid extraction and liquid chromatography mass spectrometry - Forensic entomotoxicology using an in vivo rat brain model

AU - Bushby, Sarah K.

AU - Thomas, Nicky

AU - Priemel, Petra A.

AU - Coulter, Carolyn V.

AU - Rades, Thomas

AU - Kieser, Jules A.

N1 - Copyright © 2012 Elsevier B.V. All rights reserved.

PY - 2012

Y1 - 2012

N2 - The aim of this study was to examine the potential forensic utilisation of blowfly larvae (Diptera: Calliphoridae) as an alternative toxicological specimen for the detection of the psychotropic model drug methylphenidate (MPH). MPH was extracted from biological matrices (rat brain, serum and Calliphorid larvae) by liquid-liquid extraction with recovery of >80%, and quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS assay was validated for entomotoxicological use and initially applied to male Sprague-Dawley rats (n=6) that were dosed with MPH (20mg/kg) ante-mortem. MPH could be detected in Calliphorid larvae (n=15) reared on the rat brains at 3.2±1.6ng/g. Secondly, MPH-spiked porcine brain tissue (450mg/kg) was used to investigate drug concentration in larvae over a period of 72h. After larvae had feed for 60h, MPH was detected at 19.8±1.4µg/g in the feeding larvae and at 3.5±0.1µg/g in the MPH-spiked porcine brain tissue. It could be advantageous to use Calliphorid larvae as an alternative toxicological specimen to detect alkaline labile drugs, such as MPH.

AB - The aim of this study was to examine the potential forensic utilisation of blowfly larvae (Diptera: Calliphoridae) as an alternative toxicological specimen for the detection of the psychotropic model drug methylphenidate (MPH). MPH was extracted from biological matrices (rat brain, serum and Calliphorid larvae) by liquid-liquid extraction with recovery of >80%, and quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS assay was validated for entomotoxicological use and initially applied to male Sprague-Dawley rats (n=6) that were dosed with MPH (20mg/kg) ante-mortem. MPH could be detected in Calliphorid larvae (n=15) reared on the rat brains at 3.2±1.6ng/g. Secondly, MPH-spiked porcine brain tissue (450mg/kg) was used to investigate drug concentration in larvae over a period of 72h. After larvae had feed for 60h, MPH was detected at 19.8±1.4µg/g in the feeding larvae and at 3.5±0.1µg/g in the MPH-spiked porcine brain tissue. It could be advantageous to use Calliphorid larvae as an alternative toxicological specimen to detect alkaline labile drugs, such as MPH.

U2 - 10.1016/j.jpba.2012.06.024

DO - 10.1016/j.jpba.2012.06.024

M3 - Journal article

C2 - 22795309

VL - 70

SP - 456

EP - 461

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

ER -

ID: 40849053