Determination of methylphenidate in Calliphorid larvae by liquid-liquid extraction and liquid chromatography mass spectrometry - Forensic entomotoxicology using an in vivo rat brain model
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Determination of methylphenidate in Calliphorid larvae by liquid-liquid extraction and liquid chromatography mass spectrometry - Forensic entomotoxicology using an in vivo rat brain model. / Bushby, Sarah K.; Thomas, Nicky; Priemel, Petra A.; Coulter, Carolyn V.; Rades, Thomas; Kieser, Jules A.
In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 70, 2012, p. 456-61.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Determination of methylphenidate in Calliphorid larvae by liquid-liquid extraction and liquid chromatography mass spectrometry - Forensic entomotoxicology using an in vivo rat brain model
AU - Bushby, Sarah K.
AU - Thomas, Nicky
AU - Priemel, Petra A.
AU - Coulter, Carolyn V.
AU - Rades, Thomas
AU - Kieser, Jules A.
N1 - Copyright © 2012 Elsevier B.V. All rights reserved.
PY - 2012
Y1 - 2012
N2 - The aim of this study was to examine the potential forensic utilisation of blowfly larvae (Diptera: Calliphoridae) as an alternative toxicological specimen for the detection of the psychotropic model drug methylphenidate (MPH). MPH was extracted from biological matrices (rat brain, serum and Calliphorid larvae) by liquid-liquid extraction with recovery of >80%, and quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS assay was validated for entomotoxicological use and initially applied to male Sprague-Dawley rats (n=6) that were dosed with MPH (20mg/kg) ante-mortem. MPH could be detected in Calliphorid larvae (n=15) reared on the rat brains at 3.2±1.6ng/g. Secondly, MPH-spiked porcine brain tissue (450mg/kg) was used to investigate drug concentration in larvae over a period of 72h. After larvae had feed for 60h, MPH was detected at 19.8±1.4µg/g in the feeding larvae and at 3.5±0.1µg/g in the MPH-spiked porcine brain tissue. It could be advantageous to use Calliphorid larvae as an alternative toxicological specimen to detect alkaline labile drugs, such as MPH.
AB - The aim of this study was to examine the potential forensic utilisation of blowfly larvae (Diptera: Calliphoridae) as an alternative toxicological specimen for the detection of the psychotropic model drug methylphenidate (MPH). MPH was extracted from biological matrices (rat brain, serum and Calliphorid larvae) by liquid-liquid extraction with recovery of >80%, and quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS assay was validated for entomotoxicological use and initially applied to male Sprague-Dawley rats (n=6) that were dosed with MPH (20mg/kg) ante-mortem. MPH could be detected in Calliphorid larvae (n=15) reared on the rat brains at 3.2±1.6ng/g. Secondly, MPH-spiked porcine brain tissue (450mg/kg) was used to investigate drug concentration in larvae over a period of 72h. After larvae had feed for 60h, MPH was detected at 19.8±1.4µg/g in the feeding larvae and at 3.5±0.1µg/g in the MPH-spiked porcine brain tissue. It could be advantageous to use Calliphorid larvae as an alternative toxicological specimen to detect alkaline labile drugs, such as MPH.
U2 - 10.1016/j.jpba.2012.06.024
DO - 10.1016/j.jpba.2012.06.024
M3 - Journal article
C2 - 22795309
VL - 70
SP - 456
EP - 461
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
SN - 0731-7085
ER -
ID: 40849053