Combination of LC-ICP-MS, LC-MS and NMR for investigation of the oxidative degradation of selenomethionine
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Combination of LC-ICP-MS, LC-MS and NMR for investigation of the oxidative degradation of selenomethionine. / Gammelgaard, B.; Cornett, Claus; Olsen, J.; Bendahl, L.; Hansen, S. H.
In: Talanta, Vol. 59, No. 6, 2003, p. 1165-1171.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Combination of LC-ICP-MS, LC-MS and NMR for investigation of the oxidative degradation of selenomethionine
AU - Gammelgaard, B.
AU - Cornett, Claus
AU - Olsen, J.
AU - Bendahl, L.
AU - Hansen, S. H.
PY - 2003
Y1 - 2003
N2 - Selenomethionine (SeMet) was oxidized by heating an acidic solution with hydrogen peroxide. Samples were taken before and during the oxidation process. The oxidation products were separated by cation exchange chromatography followed by ICP-MS detection to identify the selenium containing compounds as well as electrospray ionization MS detection to determine the masses of the degradation products. Furthermore, the samples were analyzed by Se-77-NMR. The first appearing degradation product was selenomethionine selenoxide, which was converted via the deaminated selenoxide to methane seleninic acid and selenite. (C) 2003 Elsevier Science B.V. All rights reserved.
AB - Selenomethionine (SeMet) was oxidized by heating an acidic solution with hydrogen peroxide. Samples were taken before and during the oxidation process. The oxidation products were separated by cation exchange chromatography followed by ICP-MS detection to identify the selenium containing compounds as well as electrospray ionization MS detection to determine the masses of the degradation products. Furthermore, the samples were analyzed by Se-77-NMR. The first appearing degradation product was selenomethionine selenoxide, which was converted via the deaminated selenoxide to methane seleninic acid and selenite. (C) 2003 Elsevier Science B.V. All rights reserved.
KW - selenomethionine selenoxide selenium speciation mass-spectrometric detection selenium chromatography acid
M3 - Tidsskriftartikel
VL - 59
SP - 1165
EP - 1171
JO - Talanta
JF - Talanta
SN - 0039-9140
IS - 6
ER -
ID: 38061314