Characterisation of extracts of Hypericum perforatum L. using an on-line HPLC system with UV/visible and fluorescence detection prior to and after photochemical conversion of the effluent

Research output: Contribution to journalJournal articlepeer-review

Standard

Characterisation of extracts of Hypericum perforatum L. using an on-line HPLC system with UV/visible and fluorescence detection prior to and after photochemical conversion of the effluent. / Jensen, A. G.; Cornett, Claus; Gudiksen, L.; Hansen, S. H.

In: Phytochemical Analysis, Vol. 11, No. 6, 2000, p. 387-394.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Jensen, AG, Cornett, C, Gudiksen, L & Hansen, SH 2000, 'Characterisation of extracts of Hypericum perforatum L. using an on-line HPLC system with UV/visible and fluorescence detection prior to and after photochemical conversion of the effluent', Phytochemical Analysis, vol. 11, no. 6, pp. 387-394.

APA

Jensen, A. G., Cornett, C., Gudiksen, L., & Hansen, S. H. (2000). Characterisation of extracts of Hypericum perforatum L. using an on-line HPLC system with UV/visible and fluorescence detection prior to and after photochemical conversion of the effluent. Phytochemical Analysis, 11(6), 387-394.

Vancouver

Jensen AG, Cornett C, Gudiksen L, Hansen SH. Characterisation of extracts of Hypericum perforatum L. using an on-line HPLC system with UV/visible and fluorescence detection prior to and after photochemical conversion of the effluent. Phytochemical Analysis. 2000;11(6):387-394.

Author

Jensen, A. G. ; Cornett, Claus ; Gudiksen, L. ; Hansen, S. H. / Characterisation of extracts of Hypericum perforatum L. using an on-line HPLC system with UV/visible and fluorescence detection prior to and after photochemical conversion of the effluent. In: Phytochemical Analysis. 2000 ; Vol. 11, No. 6. pp. 387-394.

Bibtex

@article{4200439c5420418cbeb172be3de05d61,
title = "Characterisation of extracts of Hypericum perforatum L. using an on-line HPLC system with UV/visible and fluorescence detection prior to and after photochemical conversion of the effluent",
abstract = "An HPLC system designed for the simultaneous determination of the four naphthodianthrone derivatives, protopseudohypericin, pseudohypericin, protohypericin and hypericin, in extracts of Hypericum perforatum L, has been developed. The on-line system permits quantification of the compounds by spectrophotometric detection at 590 nm as well as by fluorescence detection at 470/590 nm, Since protopseudohypericin and protohypericin are converted by exposure to light into pseudohypericin and hypericin, respectively, detection was performed before as well as after photochemical conversion in the on-line system. Verification of the peak identities was made using MS and diode-array detection. The purity of the hypericin reference material was determined by means of quantitative NMR using phenoxyacetic acid as internal standard. Several commercial extracts were characterised regarding the content of the individual naphthodianthrone derivatives using the on-line HPLC system, and the results showed the presence of similar relative amounts of naphthodianthrone derivatives in the investigated extracts. The largest total amount of hypericins in extracts of H. perforatum was obtained using detection at 590 nm after the effluent was illuminated with UV light at 254 nm for 1 min. The apparent content of hypericins was dependent on the mode of detection, and it is therefore of importance to state carefully the mode of detection in any analytical method in order to use the method for reproducible standardisation procedures. Copyright (C) 2000 John Wiley & Sons, Ltd.",
keywords = "quantitative NMR photochemical derivatisation specific absorbance hypericins Hypericum perforatum st-johns-wort antidepressant activity",
author = "Jensen, {A. G.} and Claus Cornett and L. Gudiksen and Hansen, {S. H.}",
year = "2000",
language = "Udefineret/Ukendt",
volume = "11",
pages = "387--394",
journal = "Phytochemical Analysis",
issn = "0958-0344",
publisher = "JohnWiley & Sons Ltd",
number = "6",

}

RIS

TY - JOUR

T1 - Characterisation of extracts of Hypericum perforatum L. using an on-line HPLC system with UV/visible and fluorescence detection prior to and after photochemical conversion of the effluent

AU - Jensen, A. G.

AU - Cornett, Claus

AU - Gudiksen, L.

AU - Hansen, S. H.

PY - 2000

Y1 - 2000

N2 - An HPLC system designed for the simultaneous determination of the four naphthodianthrone derivatives, protopseudohypericin, pseudohypericin, protohypericin and hypericin, in extracts of Hypericum perforatum L, has been developed. The on-line system permits quantification of the compounds by spectrophotometric detection at 590 nm as well as by fluorescence detection at 470/590 nm, Since protopseudohypericin and protohypericin are converted by exposure to light into pseudohypericin and hypericin, respectively, detection was performed before as well as after photochemical conversion in the on-line system. Verification of the peak identities was made using MS and diode-array detection. The purity of the hypericin reference material was determined by means of quantitative NMR using phenoxyacetic acid as internal standard. Several commercial extracts were characterised regarding the content of the individual naphthodianthrone derivatives using the on-line HPLC system, and the results showed the presence of similar relative amounts of naphthodianthrone derivatives in the investigated extracts. The largest total amount of hypericins in extracts of H. perforatum was obtained using detection at 590 nm after the effluent was illuminated with UV light at 254 nm for 1 min. The apparent content of hypericins was dependent on the mode of detection, and it is therefore of importance to state carefully the mode of detection in any analytical method in order to use the method for reproducible standardisation procedures. Copyright (C) 2000 John Wiley & Sons, Ltd.

AB - An HPLC system designed for the simultaneous determination of the four naphthodianthrone derivatives, protopseudohypericin, pseudohypericin, protohypericin and hypericin, in extracts of Hypericum perforatum L, has been developed. The on-line system permits quantification of the compounds by spectrophotometric detection at 590 nm as well as by fluorescence detection at 470/590 nm, Since protopseudohypericin and protohypericin are converted by exposure to light into pseudohypericin and hypericin, respectively, detection was performed before as well as after photochemical conversion in the on-line system. Verification of the peak identities was made using MS and diode-array detection. The purity of the hypericin reference material was determined by means of quantitative NMR using phenoxyacetic acid as internal standard. Several commercial extracts were characterised regarding the content of the individual naphthodianthrone derivatives using the on-line HPLC system, and the results showed the presence of similar relative amounts of naphthodianthrone derivatives in the investigated extracts. The largest total amount of hypericins in extracts of H. perforatum was obtained using detection at 590 nm after the effluent was illuminated with UV light at 254 nm for 1 min. The apparent content of hypericins was dependent on the mode of detection, and it is therefore of importance to state carefully the mode of detection in any analytical method in order to use the method for reproducible standardisation procedures. Copyright (C) 2000 John Wiley & Sons, Ltd.

KW - quantitative NMR photochemical derivatisation specific absorbance hypericins Hypericum perforatum st-johns-wort antidepressant activity

M3 - Tidsskriftartikel

VL - 11

SP - 387

EP - 394

JO - Phytochemical Analysis

JF - Phytochemical Analysis

SN - 0958-0344

IS - 6

ER -

ID: 38061523