Caracemide, a site-specific irreversible inhibitor of protein R1 of Escherichia coli ribonucleotide reductase
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Caracemide, a site-specific irreversible inhibitor of protein R1 of Escherichia coli ribonucleotide reductase. / Larsen, I. K.; Cornett, Claus; Karlsson, M.; Sahlin, M.; Sjoberg, B. M.
In: Journal of Biological Chemistry, Vol. 267, No. 18, 1992, p. 12627-12631.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Caracemide, a site-specific irreversible inhibitor of protein R1 of Escherichia coli ribonucleotide reductase
AU - Larsen, I. K.
AU - Cornett, Claus
AU - Karlsson, M.
AU - Sahlin, M.
AU - Sjoberg, B. M.
PY - 1992
Y1 - 1992
N2 - The anticancer drug caracemide, N-acetyl-N,O-di(methylcarbamoyl)hydroxylamine, and one of its degradation products, N-acetyl-O-methylcarbamoyl-hydroxylamine, were found to inhibit the enzyme ribonucleotide reductase of Escherichia coli by specific interaction with its larger component protein R1. No effect on the smaller protein R2 was observed. The effect of the degradation product was about 30 times lower than that of caracemide itself. The caracemide inactivation of R1 is irreversible, with an apparent second-order rate constant of 150 M-1 s-1. The R1R2 holoenzyme was approximately 30 times more sensitive to caracemide inactivation than the isolated R1 protein. The ribonucleotide reductase substrates were potent competitors of the caracemide inhibition, with a K(diss) for GDP binding to R1 of 80-mu-M. The reducing agent dithiothreitol was also found to be a potent competitor of caracemide inactivation. These results indicate that caracemide inactivates R1 by covalent modification at the substrate-binding site. By analogy with the known interaction between caracemide and acetylcholinesterase or choline acetyltransferase, we propose that the modification of R1 occurs at an activated cysteine or serine residue in the active site of the enzyme.
AB - The anticancer drug caracemide, N-acetyl-N,O-di(methylcarbamoyl)hydroxylamine, and one of its degradation products, N-acetyl-O-methylcarbamoyl-hydroxylamine, were found to inhibit the enzyme ribonucleotide reductase of Escherichia coli by specific interaction with its larger component protein R1. No effect on the smaller protein R2 was observed. The effect of the degradation product was about 30 times lower than that of caracemide itself. The caracemide inactivation of R1 is irreversible, with an apparent second-order rate constant of 150 M-1 s-1. The R1R2 holoenzyme was approximately 30 times more sensitive to caracemide inactivation than the isolated R1 protein. The ribonucleotide reductase substrates were potent competitors of the caracemide inhibition, with a K(diss) for GDP binding to R1 of 80-mu-M. The reducing agent dithiothreitol was also found to be a potent competitor of caracemide inactivation. These results indicate that caracemide inactivates R1 by covalent modification at the substrate-binding site. By analogy with the known interaction between caracemide and acetylcholinesterase or choline acetyltransferase, we propose that the modification of R1 occurs at an activated cysteine or serine residue in the active site of the enzyme.
KW - antitumor agent caracemide choline-acetyltransferase active-site triphosphate reductase diphosphate reductase phase-i inactivation mechanism hydroxyurea cisplatin
M3 - Tidsskriftartikel
VL - 267
SP - 12627
EP - 12631
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 18
ER -
ID: 38061790