Bovine Milk-Derived Emulsifiers Increase Triglyceride Absorption in Newborn Formula-Fed Pigs
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Bovine Milk-Derived Emulsifiers Increase Triglyceride Absorption in Newborn Formula-Fed Pigs. / Knudsen, Kristine Bach Korsholm; Heerup, Christine; Jensen, Tine Røngaard Stange; Geng, Xiaolu; Drachmann, Nikolaj; Nordby, Pernille; Jeppesen, Palle Bekker; Ifaoui, Inge; Müllertz, Anette; Sangild, Per Torp; Ostenfeld, Marie Stampe; Thymann, Thomas.
In: Nutrients, Vol. 13, No. 2, 410, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Bovine Milk-Derived Emulsifiers Increase Triglyceride Absorption in Newborn Formula-Fed Pigs
AU - Knudsen, Kristine Bach Korsholm
AU - Heerup, Christine
AU - Jensen, Tine Røngaard Stange
AU - Geng, Xiaolu
AU - Drachmann, Nikolaj
AU - Nordby, Pernille
AU - Jeppesen, Palle Bekker
AU - Ifaoui, Inge
AU - Müllertz, Anette
AU - Sangild, Per Torp
AU - Ostenfeld, Marie Stampe
AU - Thymann, Thomas
PY - 2021
Y1 - 2021
N2 - Efficient lipid digestion in formula-fed infants is required to ensure the availability of fatty acids for normal organ development. Previous studies suggest that the efficiency of lipid digestion may depend on whether lipids are emulsified with soy lecithin or fractions derived from bovine milk. This study, therefore, aimed to determine whether emulsification with bovine milk-derived emulsifiers or soy lecithin (SL) influenced lipid digestion in vitro and in vivo. Lipid digestibility was determined in vitro in oil-in-water emulsions using four different milk-derived emulsifiers or SL, and the ultrastructural appearance of the emulsions was assessed using electron microscopy. Subsequently, selected emulsions were added to a base diet and fed to preterm neonatal piglets. Initially, preterm pigs equipped with an ileostomy were fed experimental formulas for seven days and stoma output was collected quantitatively. Next, lipid absorption kinetics was studied in preterm pigs given pure emulsions. Finally, complete formulas with different emulsions were fed for four days, and the post-bolus plasma triglyceride level was determined. Milk-derived emulsifiers (containing protein and phospholipids from milk fat globule membranes and extracellular vesicles) showed increased effects on fat digestion compared to SL in an in vitro digestion model. Further, milk-derived emulsifiers significantly increased the digestion of triglyceride in the preterm piglet model compared with SL. Ultra-structural images indicated a more regular and smooth surface of fat droplets emulsified with milk-derived emulsifiers relative to SL. We conclude that, relative to SL, milk-derived emulsifiers lead to a different surface ultrastructure on the lipid droplets, and increase lipid digestion.
AB - Efficient lipid digestion in formula-fed infants is required to ensure the availability of fatty acids for normal organ development. Previous studies suggest that the efficiency of lipid digestion may depend on whether lipids are emulsified with soy lecithin or fractions derived from bovine milk. This study, therefore, aimed to determine whether emulsification with bovine milk-derived emulsifiers or soy lecithin (SL) influenced lipid digestion in vitro and in vivo. Lipid digestibility was determined in vitro in oil-in-water emulsions using four different milk-derived emulsifiers or SL, and the ultrastructural appearance of the emulsions was assessed using electron microscopy. Subsequently, selected emulsions were added to a base diet and fed to preterm neonatal piglets. Initially, preterm pigs equipped with an ileostomy were fed experimental formulas for seven days and stoma output was collected quantitatively. Next, lipid absorption kinetics was studied in preterm pigs given pure emulsions. Finally, complete formulas with different emulsions were fed for four days, and the post-bolus plasma triglyceride level was determined. Milk-derived emulsifiers (containing protein and phospholipids from milk fat globule membranes and extracellular vesicles) showed increased effects on fat digestion compared to SL in an in vitro digestion model. Further, milk-derived emulsifiers significantly increased the digestion of triglyceride in the preterm piglet model compared with SL. Ultra-structural images indicated a more regular and smooth surface of fat droplets emulsified with milk-derived emulsifiers relative to SL. We conclude that, relative to SL, milk-derived emulsifiers lead to a different surface ultrastructure on the lipid droplets, and increase lipid digestion.
KW - Absorption
KW - Emulsions
KW - Fat
KW - Gastric lipase
KW - Intestine
KW - Milk
KW - Preterm neonates
KW - Soy lecithin
KW - Vegetable oil
U2 - 10.3390/nu13020410
DO - 10.3390/nu13020410
M3 - Journal article
C2 - 33525418
AN - SCOPUS:85099969620
VL - 13
JO - Nutrients
JF - Nutrients
SN - 2072-6643
IS - 2
M1 - 410
ER -
ID: 257920325