Adding a Gastric Step to the Intestinal In Vitro Digestion Model Improves the Prediction of Pharmacokinetic Data in Beagle Dogs of Two Lipid-Based Drug Delivery Systems
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Adding a Gastric Step to the Intestinal In Vitro Digestion Model Improves the Prediction of Pharmacokinetic Data in Beagle Dogs of Two Lipid-Based Drug Delivery Systems. / Klitgaard, Mette; Beilles, Stephane; Sassene, Philip Jonas; Berthelsen, Ragna; Mullertz, Anette.
In: Molecular Pharmaceutics, Vol. 17, No. 9, 2020, p. 3214-3222.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Adding a Gastric Step to the Intestinal In Vitro Digestion Model Improves the Prediction of Pharmacokinetic Data in Beagle Dogs of Two Lipid-Based Drug Delivery Systems
AU - Klitgaard, Mette
AU - Beilles, Stephane
AU - Sassene, Philip Jonas
AU - Berthelsen, Ragna
AU - Mullertz, Anette
PY - 2020
Y1 - 2020
N2 - Drug release from a lipid-based drug delivery system (LbDDS) is typically studied in vitro using a one-step intestinal digestion model. However, lately the importance of incorporating gastric digestion has been stressed. The aim of the present study was to compare a two-step gastro-intestinal (GI) in vitro digestion model to the commonly used one-step intestinal digestion model. The models were evaluated by studying release of the model drug A1260 from two LbDDSs (F-I and F-II), for which in vivo pharmacokinetic data from oral administration to beagle dogs were available. The amount of A1260 recovered in the aqueous phases during and after the GI digestion of F-I and F-II was related to the Cmax and AUC0- 48h of the plasma concentration-time profiles of each formulation and produced a rank order in vitroin vivo (IVIV) relation. In comparison, a similar IVIV rank ordering was obtained when relating the amount of A1260 recovered in the aqueous phase prior (t = 0 min), and following 15 min of intestinal digestion, to the plasma concentration-time profiles. However, after 60 min of intestinal digestion, the LbDDSs performed equally in the one-step in vitro digestion model, contrary to what was observed in the two-step digestion model, and in vivo. As the GI digestion model produced a clearer distinction in terms of LbDDS rank ordering of the two LbDDSs, compared to the intestinal digestion model, it was found to be a promising in vitro model to study and estimate the LbDDS behavior in vivo.
AB - Drug release from a lipid-based drug delivery system (LbDDS) is typically studied in vitro using a one-step intestinal digestion model. However, lately the importance of incorporating gastric digestion has been stressed. The aim of the present study was to compare a two-step gastro-intestinal (GI) in vitro digestion model to the commonly used one-step intestinal digestion model. The models were evaluated by studying release of the model drug A1260 from two LbDDSs (F-I and F-II), for which in vivo pharmacokinetic data from oral administration to beagle dogs were available. The amount of A1260 recovered in the aqueous phases during and after the GI digestion of F-I and F-II was related to the Cmax and AUC0- 48h of the plasma concentration-time profiles of each formulation and produced a rank order in vitroin vivo (IVIV) relation. In comparison, a similar IVIV rank ordering was obtained when relating the amount of A1260 recovered in the aqueous phase prior (t = 0 min), and following 15 min of intestinal digestion, to the plasma concentration-time profiles. However, after 60 min of intestinal digestion, the LbDDSs performed equally in the one-step in vitro digestion model, contrary to what was observed in the two-step digestion model, and in vivo. As the GI digestion model produced a clearer distinction in terms of LbDDS rank ordering of the two LbDDSs, compared to the intestinal digestion model, it was found to be a promising in vitro model to study and estimate the LbDDS behavior in vivo.
KW - gastro-intestinal digestion
KW - in vitro
KW - in vivo
KW - lipid-based drug delivery system
KW - oral biopharmaceutical tools (OrBiTo)
KW - WATER-SOLUBLE DRUGS
KW - LIPOLYSIS MODEL
KW - GASTROINTESTINAL LIPOLYSIS
KW - VIVO PERFORMANCE
KW - FORMULATIONS
KW - LIPASES
KW - TESTS
KW - BIOAVAILABILITY
KW - ESTABLISHMENT
KW - DISSOLUTION
U2 - 10.1021/acs.molpharmaceut.0c00307
DO - 10.1021/acs.molpharmaceut.0c00307
M3 - Journal article
C2 - 32662647
VL - 17
SP - 3214
EP - 3222
JO - Molecular Pharmaceutics
JF - Molecular Pharmaceutics
SN - 1543-8384
IS - 9
ER -
ID: 254461076