A comparison of pseudo-ternary diagrams of aqueous mixtures of Quil A, cholesterol and phospholipid prepared by lipid-film hydration and dialysis

Research output: Contribution to journalJournal articlepeer-review

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A comparison of pseudo-ternary diagrams of aqueous mixtures of Quil A, cholesterol and phospholipid prepared by lipid-film hydration and dialysis. / Demana, P. H.; Davies, N. M.; Berger, B.; Vosgerau, U.; Rades, Thomas.

In: Journal of Pharmacy and Pharmacology, Vol. 56, No. 5, 05.2004, p. 573-580.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Demana, PH, Davies, NM, Berger, B, Vosgerau, U & Rades, T 2004, 'A comparison of pseudo-ternary diagrams of aqueous mixtures of Quil A, cholesterol and phospholipid prepared by lipid-film hydration and dialysis', Journal of Pharmacy and Pharmacology, vol. 56, no. 5, pp. 573-580. https://doi.org/10.1211/0022357023259

APA

Demana, P. H., Davies, N. M., Berger, B., Vosgerau, U., & Rades, T. (2004). A comparison of pseudo-ternary diagrams of aqueous mixtures of Quil A, cholesterol and phospholipid prepared by lipid-film hydration and dialysis. Journal of Pharmacy and Pharmacology, 56(5), 573-580. https://doi.org/10.1211/0022357023259

Vancouver

Demana PH, Davies NM, Berger B, Vosgerau U, Rades T. A comparison of pseudo-ternary diagrams of aqueous mixtures of Quil A, cholesterol and phospholipid prepared by lipid-film hydration and dialysis. Journal of Pharmacy and Pharmacology. 2004 May;56(5):573-580. https://doi.org/10.1211/0022357023259

Author

Demana, P. H. ; Davies, N. M. ; Berger, B. ; Vosgerau, U. ; Rades, Thomas. / A comparison of pseudo-ternary diagrams of aqueous mixtures of Quil A, cholesterol and phospholipid prepared by lipid-film hydration and dialysis. In: Journal of Pharmacy and Pharmacology. 2004 ; Vol. 56, No. 5. pp. 573-580.

Bibtex

@article{292f28c39baf41aaa0cd6a6babde9b09,
title = "A comparison of pseudo-ternary diagrams of aqueous mixtures of Quil A, cholesterol and phospholipid prepared by lipid-film hydration and dialysis",
abstract = "Pseudo-ternary diagrams for Quil A, phospholipid (phosphatidylcholine (PC) or phosphatidylethanolamine (PE)) and cholesterol were established in order to identify combinations that result in the formation of immune-stimulating complex (ISCOM) matrices and other colloidal structures produced by these three components in aqueous systems following lipid-film hydration or dialysis (methods that can be used to produce ISCOMs). In addition, the effect of equilibration time (1 month at 4 °C) on the structures formed by the various combinations of the three components was investigated. Depending on the ratio of Quil A, cholesterol and phospholipid, different colloidal particles, including ISCOM matrices, liposomes and ring-like micelles, were found irrespective of the preparation method used. In contrast, worm-like micelles were only observed in systems prepared by lipid-film hydration. For samples prepared by dialysis, ISCOM matrices were predominantly found near the Quil A apex of the pseudo-ternary diagram (> 50% Quil A). On the other hand, for samples prepared by lipid-film hydration, ISCOM matrices were predominantly found near the phospholipid apex of the pseudo-ternary diagram (> 50% phospholipid). The regions in the pseudo-ternary diagrams in which ISCOM matrices were observed increased following an extended equilibration time, particularly for samples prepared by lipid-film hydration. Differences were also observed between pseudoternary diagrams prepared using either PE or PC as phospholipids.",
author = "Demana, {P. H.} and Davies, {N. M.} and B. Berger and U. Vosgerau and Thomas Rades",
year = "2004",
month = may,
doi = "10.1211/0022357023259",
language = "English",
volume = "56",
pages = "573--580",
journal = "Journal of Pharmacy and Pharmacology",
issn = "0022-3573",
publisher = "JohnWiley & Sons Ltd",
number = "5",

}

RIS

TY - JOUR

T1 - A comparison of pseudo-ternary diagrams of aqueous mixtures of Quil A, cholesterol and phospholipid prepared by lipid-film hydration and dialysis

AU - Demana, P. H.

AU - Davies, N. M.

AU - Berger, B.

AU - Vosgerau, U.

AU - Rades, Thomas

PY - 2004/5

Y1 - 2004/5

N2 - Pseudo-ternary diagrams for Quil A, phospholipid (phosphatidylcholine (PC) or phosphatidylethanolamine (PE)) and cholesterol were established in order to identify combinations that result in the formation of immune-stimulating complex (ISCOM) matrices and other colloidal structures produced by these three components in aqueous systems following lipid-film hydration or dialysis (methods that can be used to produce ISCOMs). In addition, the effect of equilibration time (1 month at 4 °C) on the structures formed by the various combinations of the three components was investigated. Depending on the ratio of Quil A, cholesterol and phospholipid, different colloidal particles, including ISCOM matrices, liposomes and ring-like micelles, were found irrespective of the preparation method used. In contrast, worm-like micelles were only observed in systems prepared by lipid-film hydration. For samples prepared by dialysis, ISCOM matrices were predominantly found near the Quil A apex of the pseudo-ternary diagram (> 50% Quil A). On the other hand, for samples prepared by lipid-film hydration, ISCOM matrices were predominantly found near the phospholipid apex of the pseudo-ternary diagram (> 50% phospholipid). The regions in the pseudo-ternary diagrams in which ISCOM matrices were observed increased following an extended equilibration time, particularly for samples prepared by lipid-film hydration. Differences were also observed between pseudoternary diagrams prepared using either PE or PC as phospholipids.

AB - Pseudo-ternary diagrams for Quil A, phospholipid (phosphatidylcholine (PC) or phosphatidylethanolamine (PE)) and cholesterol were established in order to identify combinations that result in the formation of immune-stimulating complex (ISCOM) matrices and other colloidal structures produced by these three components in aqueous systems following lipid-film hydration or dialysis (methods that can be used to produce ISCOMs). In addition, the effect of equilibration time (1 month at 4 °C) on the structures formed by the various combinations of the three components was investigated. Depending on the ratio of Quil A, cholesterol and phospholipid, different colloidal particles, including ISCOM matrices, liposomes and ring-like micelles, were found irrespective of the preparation method used. In contrast, worm-like micelles were only observed in systems prepared by lipid-film hydration. For samples prepared by dialysis, ISCOM matrices were predominantly found near the Quil A apex of the pseudo-ternary diagram (> 50% Quil A). On the other hand, for samples prepared by lipid-film hydration, ISCOM matrices were predominantly found near the phospholipid apex of the pseudo-ternary diagram (> 50% phospholipid). The regions in the pseudo-ternary diagrams in which ISCOM matrices were observed increased following an extended equilibration time, particularly for samples prepared by lipid-film hydration. Differences were also observed between pseudoternary diagrams prepared using either PE or PC as phospholipids.

UR - http://www.scopus.com/inward/record.url?scp=2342553509&partnerID=8YFLogxK

U2 - 10.1211/0022357023259

DO - 10.1211/0022357023259

M3 - Journal article

C2 - 15142333

AN - SCOPUS:2342553509

VL - 56

SP - 573

EP - 580

JO - Journal of Pharmacy and Pharmacology

JF - Journal of Pharmacy and Pharmacology

SN - 0022-3573

IS - 5

ER -

ID: 299428765