Birger Brodin Larsen
Professor
CNS Drug Delivery and Barrier Modelling
Universitetsparken 2
2100 København Ø
- 2024
- Published
Application of a new MDCKII-MDR1 cell model to measure the extent of drug distribution in vitro at equilibrium for prediction of in vivo unbound brain-to-plasma drug distribution
Langthaler, Kristine, Jones, C. R., Saaby, Lasse, Bundgaard, C. & Larsen, Birger Brodin, 2024, In: Fluids and Barriers of the CNS. 21, 1, 15 p., 11.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus
Nielsen, A. K. R., Lilieholm-Røngren, L., Schmid, B., Holst, B., Larsen, Birger Brodin & Saaby, Lasse, 2024, In: Stem Cell Research. 76, 5 p., 103348.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
The solute carrier SLC7A1 may act as a protein transporter at the blood-brain barrier
Kurtyka, M., Wessely, F., Bau, S., Ifie, E., He, L., de Wit, N. M., Pedersen, A. B. V., Keller, M., Webber, C., de Vries, H. E., Ansorge, O., Betsholtz, C., De Bock, M., Chaves, C., Brodin, B., Nielsen, M. S., Neuhaus, W., Bell, R. D., Letoha, T., Meyer, A. H. & 7 others, , 2024, In: European Journal of Cell Biology. 103, 2, 14 p., 151406.Research output: Contribution to journal › Journal article › Research › peer-review
- 2023
- Published
Apicobasal transferrin receptor localization and trafficking in brain capillary endothelial cells
Nielsen, S. S. E., Holst, M. R., Langthaler, Kristine, Bruun, E. H., Larsen, Birger Brodin & Nielsen, M. S., 2023, In: Fluids and Barriers of the CNS. 20, 1, 15 p., 2.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Assessing extent of brain penetration in vivo (Kp,uu,brain) in Göttingen minipig using a diverse set of reference drugs
Langthaler, Kristine, Jones, C. R., Larsen, Birger Brodin & Bundgaard, C., 2023, In: European Journal of Pharmaceutical Sciences. 190, 11 p., 106554.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Characterization of an iPSC-based barrier model for blood-brain barrier investigations using the SBAD0201 stem cell line
Ozgür, Burak, Puris, E., Brachner, A., Appelt-Menzel, A., Oerter, S., Balzer, V., Holst, M. R., Christiansen, R. F., Hyldig, K., Buckley, S. T., Kristensen, Mie, Auriola, S., Jensen, A., Fricker, G., Nielsen, M. S., Neuhaus, W. & Larsen, Birger Brodin, 2023, In: Fluids and Barriers of the CNS. 20, 1, 15 p., 96.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Subcellular trafficking and transcytosis efficacy of different receptor types for therapeutic antibody delivery at the blood‒brain barrier
Holst, M. R., de Wit, N. M., Ozgür, Burak, Brachner, A., Hyldig, K., Appelt-Menzel, A., Sleven, H., Cader, Z., de Vries, H. E., Neuhaus, W., Jensen, A., Larsen, Birger Brodin & Nielsen, M. S., 2023, In: Fluids and Barriers of the CNS. 20, 1, 17 p., 82.Research output: Contribution to journal › Journal article › Research › peer-review
- 2022
- Published
Characterisation of intravenous pharmacokinetics in Göttingen minipig and clearance prediction using established in vitro to in vivo extrapolation methodologies
Langthaler, Kristine, Jones, C. R., Christensen, R. B., Eneberg, E., Larsen, Birger Brodin & Bundgaard, C., 2022, In: Xenobiotica. 52, 6, p. 591-607Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Highly cationic cell-penetrating peptides affect the barrier integrity and facilitates mannitol permeation in a human stem cell-based blood-brain barrier model
Frøslev, Patrick, Franzyk, Henrik, Ozgür, Burak, Larsen, Birger Brodin & Kristensen, Mie, 2022, In: European Journal of Pharmaceutical Sciences. 168, 10 p., 106054.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Hypoxia increases expression of selected blood–brain barrier transporters GLUT-1, P-gp, SLC7A5 and TFRC, while maintaining barrier integrity, in brain capillary endothelial monolayers
Ozgür, Burak, Helms, H. C. C., Tornabene, E. & Larsen, Birger Brodin, 2022, In: Fluids and Barriers of the CNS. 19, 1, 17 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Sortilin regulates blood-brain barrier integrity
Toth, A. E., Helms, H. C., Harazin, A., Johnsen, K. B., Goldeman, C., Burkhart, A., Thomsen, M. S., Kempen, P. J., Klepe, A., Lipka, D. V., Møller, P. L., Andresen, T. L., Nyegaard, M., Moos, T., Larsen, Birger Brodin & Nielsen, M. S., 2022, In: The FEBS Journal. 289, 4, p. 1062-1079Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Tanycytes control hypothalamic liraglutide uptake and its anti-obesity actions
Imbernon, M., Saponaro, C., Helms, H. C. C., Duquenne, M., Fernandois, D., Deligia, E., Denis, R. G. P., Chao, D. H. M., Rasika, S., Staels, B., Pattou, F., Pfrieger, F. W., Larsen, Birger Brodin, Luquet, S., Bonner, C. & Prevot, V., 2022, In: Cell Metabolism. 34, 7, p. 1054-1063.e7Research output: Contribution to journal › Journal article › Research › peer-review
- Published
The Cell-Penetrating Peptide Tat Facilitates Effective Internalization of PSD-95 Inhibitors Into Blood–Brain Barrier Endothelial Cells but less Efficient Permeation Across the Blood–Brain Barrier In Vitro and In Vivo
Lisa Al Humaidan, E., Lund Pedersen, S., Burkhart, A., Laurfelt Munch Rasmussen, C., Moos, T., Fuchs, P., Alves Fernandes, E. F., Ozgür, Burak, Strømgaard, K., Bach, Anders, Larsen, Birger Brodin & Kristensen, Mie, 2022, In: Frontiers in Drug Delivery - CNS Drug Delivery. 2, 17 p., 854703.Research output: Contribution to journal › Journal article › Research › peer-review
- 2021
- Published
Tanycytes Control the Hypothalamic Uptake and Metabolic Actions of Liraglutide
Imbernon, M., Saponaro, C., Helms, H. C. C., Zubiaga, L., Bitsi, S., Duquenne, M., Tomas, A., Chen, S., Salem, V., Deligia, E., Gmyr, V., Denis, R., Kerr-Conte, J., Chao, D. H. M., Beiroa, D., Steals, B., Pattou, F., Pfrieger, F., Brodin, B., Jones, B. & 3 others, , Jul 2021, In: Glia. 69, S1, p. E237-E237Research output: Contribution to journal › Conference abstract in journal › Research
- Published
ATP induces contraction of cultured brain capillary pericytes, via activation of P2Y type purinergic receptors
Hørlyck, S., Cai, Changsi, Helms, H. C., Lauritzen, Martin & Larsen, Birger Brodin, 2021, In: American Journal of Physiology: Heart and Circulatory Physiology. 320, p. H699–H712 14 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Brain endothelial cells metabolize glutamate via glutamate dehydrogenase to replenish TCA-intermediates and produce ATP under hypoglycemic conditions
Hinca, S. B., Salcedo, C., Wagner, A., Goldeman, C., Sadat, E., Aibar, M. M. D., Maechler, P., Larsen, Birger Brodin, Aldana, Blanca & Helms, H. C. C., 2021, In: Journal of Neurochemistry. 157, 6, p. 1861-1875Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Human induced pluripotent stem cells (BIONi010-C) generate tight cell monolayers with blood-brain barrier traits and functional expression of large neutral amino acid transporter 1 (SLC7A5)
Goldeman, C., Andersen, M., Al-Robai, A., Buchholtz, T., Svane, Nana Isabella, Ozgür, Burak, Holst, B., Shusta, E., Hall, Vanessa Jane, Saaby, Lasse, Hyttel, P. & Larsen, Birger Brodin, 2021, In: European Journal of Pharmaceutical Sciences. 156, 14 p., 105577.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Precapillary sphincters and pericytes at first-order capillaries as key regulators for brain capillary perfusion
Zambach, S. A., Cai, Changsi, Helms, H. C. C., Hald, B. O., Dong, Y., Fordsmann, J. C., Nielsen, R. M., Hu, J., wbh615, wbh615, Larsen, Birger Brodin & Lauritzen, Martin, 2021, In: Proceedings of the National Academy of Sciences of the United States of America. 118, 26, e2023749118.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Screening novel CNS drug candidates for P-glycoprotein interactions using the cell line iP-gp: In vitro efflux ratios from iP-gp and MDCK-MDR1 monolayers compared to brain distribution data from mice
Ozgür, Burak, Saaby, Lasse, Janfelt, Christian, Langthaler, K., Eneberg, E., Jacobsen, A., Badolo, L., Montanari, D. & Larsen, Birger Brodin, 2021, In: European Journal of Pharmaceutics and Biopharmaceutics. 169, p. 211-219Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Transendothelial electrical resistance measurement across the blood–brain barrier: A critical review of methods
Vigh, J. P., Kincses, A., Ozgür, Burak, Walter, F. R., Santa-Maria, A. R., Valkai, S., Vastag, M., Neuhaus, W., Larsen, Birger Brodin, Dér, A. & Deli, M. A., 2021, In: Micromachines. 12, 6, 18 p., 685.Research output: Contribution to journal › Review › Research › peer-review
- 2020
- Published
IPEC-J2 rMdr1a, a New Cell Line with Functional Expression of Rat P-glycoprotein Encoded by Rat Mdr1a for Drug Screening Purposes
Saaby, Lasse, Trasborg, J., Rasmussen, M. A., Holst, B. & Larsen, Birger Brodin, 17 Jul 2020, In: Pharmaceutics. 12, 7, 17 p., 673.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Culture of brain capillary pericytes for cytosolic calcium measurements and calcium imaging studies
Hørlyck, S., Helms, H. C. C. & Larsen, Birger Brodin, May 2020, In: Journal of Visualized Experiments. 159, p. 1-9 9 p., e61253.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Culture-induced changes in mRNA expression levels of efflux and SLC-transporters in brain endothelial cells
Goldeman, C., Ozgür, Burak & Larsen, Birger Brodin, 22 Apr 2020, In: Fluids and Barriers of the CNS. 17, 32, 12 p., 32.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
The Pyrazolo[3,4-d]pyrimidine Derivative, SCO-201, Reverses Multidrug Resistance Mediated by ABCG2/BCRP
Ambjørner, S. E. B., Wiese, M., Köhler, S. C., Svindt, J., Lund, X. L., Gajhede, Michael, Saaby, Lasse, Larsen, Birger Brodin, Rump, S., Weigt, H., Brünner, N. & Stenvang, Jan, 4 Mar 2020, In: Cells. 9, 3, 22 p., 613.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Conjugation of Therapeutic PSD-95 Inhibitors to the Cell-Penetrating Peptide Tat Affects Blood-Brain Barrier Adherence, Uptake, and Permeation
Kristensen, Mie, Kucharz, Krzysztof, Alves Fernandes, Eduardo Felipe, Strømgaard, Kristian, Schallburg Nielsen, M., Cederberg Helms, H. C., Bach, Anders, Ulrikkaholm Tofte-Hansen, M., Aldana, Blanca, Lauritzen, Martin & Larsen, Birger Brodin, 2020, In: Pharmaceutics. 12, 7, 24 p., 661.Research output: Contribution to journal › Journal article › Research › peer-review
- 2019
- Published
miRNA-27a-3p and miRNA-222-3p as Novel Modulators of Phosphodiesterase 3a (PDE3A) in Cerebral Microvascular Endothelial Cells
Yasmeen, S., Kaur, S., Mirza, A. H., Larsen, Birger Brodin, Pociot, Flemming & Kruuse, Christina Rostrup, Aug 2019, In: Molecular Neurobiology. 56, 8, p. 5304–5314Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Effects of oxygen-glucose deprivation (OGD) on barrier properties and mRNA transcript levels of selected marker proteins in brain endothelial cells/astrocyte co-cultures
Tornabene, E., Helms, H. C. C., Pedersen, Stine Helene Falsig & Larsen, Birger Brodin, 2019, In: PLoS ONE. 14, 8, 20 p., e0221103.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Evaluating the involvement of cerebral microvascular endothelial Na+/K+-ATPase and Na+-K+-2Cl- co-transporter in electrolyte fluxes in an in vitro blood-brain barrier model of dehydration
Lykke, K., Assentoft, M., Hørlyck, S., Helms, H. C. C., Stoica, A., Toft-Bertelsen, Trine Lisberg, Tritsaris, Katerina, Vilhardt, Frederik, Larsen, Birger Brodin & MacAulay, Nanna, 2019, In: Journal of Cerebral Blood Flow and Metabolism. 39, 3, p. 497-512Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Two-photon fluorescence imaging: a platform to investigate cell-penetrating peptides and liposome-based nanocarriers for drug delivery across the heterogeneous BBB in vivo
Kucharz, K., Kristensen, K., Kristensen, Mie, Lund, M., Andresen, T., Larsen, Birger Brodin & Lauritzen, M., 2019, In: Journal of Cerebral Blood Flow and Metabolism. 39, p. 60 1 p.Research output: Contribution to journal › Conference abstract in journal › Research › peer-review
- 2018
- Published
Validation of reference genes for normalization of real-time quantitative PCR studies of gene expression in brain capillary endothelial cells cultured in vitro
Hersom, M., Goldeman, C., Pretzer, N. & Larsen, Birger Brodin, Dec 2018, In: Molecular and Cellular Neuroscience. 93, p. 27-35 9 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Characterization of the IPEC-J2 MDR1 (iP-gp) cell line as a tool for identification of P-gp substrates
Ozgür, Burak, Saaby, Lasse, Langthaler, K. & Larsen, Birger Brodin, 2018, In: European Journal of Pharmaceutical Sciences. 112, p. 112-121 10 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Data demonstrating the challenges of determining the kinetic parameters of P-gp mediated transport of low-water soluble substrates
Ozgür, Burak, Saaby, Lasse, Langthaler, K. & Larsen, Birger Brodin, 2018, In: Data in Brief. 16, p. 655-659 5 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Stimulation-induced increases in cerebral blood flow and local capillary vasoconstriction depend on conducted vascular responses
Cai, Changsi, Fordsmann, J. C., Jensen, S. H., Gesslein, B., wbh615, wbh615, Hald, B. O., Zambach, S. A., Larsen, Birger Brodin & Lauritzen, Martin, 2018, In: Proceedings of the National Academy of Sciences of the United States of America. 115, 25, p. E5796-E5804 9 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
The insulin receptor is expressed and functional in cultured blood-brain barrier endothelial cells, but does not mediate insulin entry from blood-to-brain
Hersom, M., Helms, H. C., Schmalz, C., Pedersen, T. Å., Buckley, S. T. & Larsen, Birger Brodin, 2018, In: American Journal of Physiology: Endocrinology and Metabolism. 315, 4, p. E531-E542Research output: Contribution to journal › Journal article › Research › peer-review
- 2017
- Published
Current research into brain barriers and the delivery of therapeutics for neurological diseases: a report on CNS barrier congress London, UK, 2017
Greenwood, J., Hammarlund-Udenaes, M., Jones, H. C., Stitt, A. W., Vandenbrouke, R. E., Romero, I. A., Campbell, M., Fricker, G., Larsen, Birger Brodin, Manninga, H., Gaillard, P. J., Schwaninger, M., Webster, C., Wicher, K. B. & Khrestchatisky, M., 7 Nov 2017, In: Fluids and Barriers of the CNS. 14, 11 p., 31.Research output: Contribution to journal › Review › Research › peer-review
- Published
A Critical View on In Vitro Analysis of P-glycoprotein (P-gp) Transport Kinetics
Saaby, Lasse & Larsen, Birger Brodin, Sep 2017, In: Journal of Pharmaceutical Sciences. 106, 9, p. 2257-2264 8 p.Research output: Contribution to journal › Review › Research › peer-review
- Published
Routes for drug translocation across the blood-brain barrier: Exploiting peptides as delivery vectors
Kristensen, Mie & Larsen, Birger Brodin, Sep 2017, In: Journal of Pharmaceutical Sciences. 106, 9, p. 2326-2334Research output: Contribution to journal › Review › Research › peer-review
- Published
Bidirectional apical-basal traffic of the cation-independent mannose-6-phosphate receptor in brain endothelial cells
Siupka, P., Hersom, M. N., Lykke-Hartmann, K., Johnsen, K. B., Thomsen, L. B., Andresen, T. L., Moos, T., Abbott, N. J. & Larsen, Birger Brodin, 1 Jan 2017, In: Journal of Cerebral Blood Flow and Metabolism. 37, 7, p. 2598-2613Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Characterization of the L-glutamate clearance pathways across the blood-brain barrier and the effect of astrocytes in an in vitro blood-brain barrier model
Helms, H. C., Aldana, Blanca, Groth, S., Jensen, M. M., Waagepetersen, Helle S., Nielsen, C. U. & Larsen, Birger Brodin, 2017, In: Journal of Cerebral Blood Flow and Metabolism. 37, 12, p. 3744–3758Research output: Contribution to journal › Journal article › Research › peer-review
- 2016
- Published
Transferrin receptor expression and role in transendothelial transport of transferrin in cultured brain endothelial monolayers
Hersom, M., Helms, H. C., Pretzer, N., Andersen, C. G., Jensen, A. I., Severin, G., Nielsen, M. S., Holm, R. & Larsen, Birger Brodin, 24 Aug 2016, In: Molecular and Cellular Neuroscience. 76, p. 59-67 9 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
In vitro blood-brain barrier permeability predictions for GABAA receptor modulating piperine analogs
Eigenmann, D. E., Dürig, C., Jähne, E. A., Smieško, M., Culot, M., Gosselet, F., Cecchelli, R., Helms, H. C. C., Larsen, Birger Brodin, Wimmer, L., Mihovilovic, M. D., Hamburger, M. & Oufir, M., Jun 2016, In: European Journal of Pharmaceutics and Biopharmaceutics. 103, p. 118-26 9 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
In vitro models of the blood-brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use
Helms, H. C. C., Abbott, N. J., Burek, M., Cecchelli, R., Couraud, P., Deli, M. A., Förster, C., Galla, H. J., Romero, I. A., Shusta, E. V., Stebbins, M. J., Vandenhaute, E., Weksler, B. & Larsen, Birger Brodin, May 2016, In: Journal of Cerebral Blood Flow and Metabolism. 36, 5, p. 862-90 29 p.Research output: Contribution to journal › Review › Research › peer-review
- Published
IPEC-J2 MDR1, a Novel High-Resistance Cell Line with Functional Expression of Human P-glycoprotein (ABCB1) for Drug Screening Studies
Saaby, Lasse, Helms, H. C. C. & Larsen, Birger Brodin, 1 Feb 2016, In: Molecular Pharmaceutics. 13, 2, p. 640-52 13 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Stroke and Drug Delivery--In Vitro Models of the Ischemic Blood-Brain Barrier
Tornabene, E. & Larsen, Birger Brodin, Feb 2016, In: Journal of Pharmaceutical Sciences. 105, 2, p. 398-405 8 p.Research output: Contribution to journal › Journal article › Research › peer-review
- 2015
- Published
The putative P-gp inhibitor telmisartan does not affect the transcellular permeability and cellular uptake of the calcium channel antagonist verapamil in the P-glycoprotein expressing cell line MDCK II MDR1
Saaby, Lasse, Tfelt-Hansen, P. & Larsen, Birger Brodin, 11 Jun 2015, In: Pharmacology Research & Perspectives. 3, 4, p. 1-9 9 p., e00151.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
In Vitro Blood-Brain Barrier Models-An Overview of Established Models and New Microfluidic Approaches
Wolff, A., Antfolk, M., Larsen, Birger Brodin & Tenje, M., 28 Jan 2015, In: Journal of Pharmaceutical Sciences. 104, 9, p. 2727-2746 20 p.Research output: Contribution to journal › Journal article › Research › peer-review
- 2014
- Published
Glutamate Efflux at the Blood-Brain Barrier: Cellular Mechanisms and Potential Clinical Relevance
Cederberg-Helms, H. C., Uhd-Nielsen, C. & Larsen, Birger Brodin, 15 Nov 2014, In: Archives of Medical Research. 45, 8, p. 639-645 7 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
An Electrically Tight In Vitro Blood-Brain Barrier Model Displays Net Brain-to-Blood Efflux of Substrates for the ABC Transporters, P-gp, Bcrp and Mrp-1
Helms, H. C., Hersom, M., Kuhlmann, L. B., Badolo, L., Nielsen, C. U. & Larsen, Birger Brodin, 17 Jun 2014, In: A A P S Journal. 16, 5, p. 1046-1055 10 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
In Vitro Membrane Permeation Studies and in Vivo Antinociception of Glycosylated Dmt(1)-DALDA Analogues
Ballet, S., Betti, C., Novoa, A., Tömböly, C., Nielsen, C. U., Helms, H. C., Lesniak, A., Kleczkowska, P., Chung, N. N., Lipkowski, A. W., Larsen, Birger Brodin, Tourwé, D. & Schiller, P. W., 10 Apr 2014, In: ACS Medicinal Chemistry Letters. 5, 4, p. 352-357 6 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
The absorptive flux of the anti-epileptic drug substance vigabatrin is carrier-mediated across Caco-2 cell monolayers
Nøhr, M. K., Hansen, S. H., Larsen, Birger Brodin, Holm, R. & Nielsen, C. U., 23 Jan 2014, In: European Journal of Pharmaceutical Sciences. 51, p. 1-10Research output: Contribution to journal › Journal article › Research › peer-review
ID: 1299175
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Design of prodrugs targeting the intestinal di/tri‐peptide transporter hPEPT1 (SLC15A1
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A bovine in vitro blood-brain barrier model under oxygenglucose deprivation (OGD) condition
Research output: Contribution to conference › Poster › Research
Published