Quantitative analysis of therapeutic proteins – University of Copenhagen

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Department of Pharmacy > Research > Section of Analytical Biosciences > Protein Analysis > Quantitative analysis ...

Quantitative analysis of therapeutic proteins

Quantifying therapeutic proteins in biological samples is an essential part of drug discovery, ADME, toxicology, pre-clinical and clinical development. It is however challenging to develop suitable methods that provide sensitive and robust results. For these tasks liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) has recently shown as a powerful technique to solve this task. We are, together with leading experts, developing and validating advanced and specialized LC-MS based methods for sensitive and robust measurement of protein therapeutics in complex biological samples.

Figure 1. Employing LC-MS/MS for analysis of mice plasma samples for use in pharmacokinetic studies.

Current projects and expertise in quantification of therapeutic proteins in the lab:

  • Extraction and purification of therapeutic proteins from complex biological samples.
  • Absolute and relative quantification of therapeutic proteins in complex biological samples.
  • Development, validation and routine analysis of bioanalytical samples of protein pharmaceuticals
  • Using and development new state-of-the-art mass spectrometric methods and workflows for analysis.
  • Bioanalysis of biopharmaceuticals in animal and human samples in PK, ADME, toxicological, preclinical- and clinical studies.

Figure 2. Schematic illustration of a standard workflow for quantification of therapeutic proteins in complex biological samples with LC-MS/MS.