Department of Pharmacy > Research > Section of Analytical Biosciences > Protein Analysis

Protein Analysis Group

In the Protein Analysis Group, we focus on in-depth and comprehensive analysis of proteins of pharmaceutical interest. This includes quantitative analysis, the characterization of protein and peptide primary structure and the higher-order structure and interactions of proteins, in addition to development of new mass spectrometric methods and workflows for studying these.

The majority of projects in the Protein Analysis Group focus on proteins that are current or potential drugs or drug targets. Therefore most projects are performed in collaboration with external academic and industrial partners, including drug development labs and pharmaceutical/biotech companies.

• Analysis of the primary structure of peptides and proteins

  Using liquid chromatography and mass spectrometry for detailed characterisation of proteins primary structure, including the nature and location of modifications and degradations.
  >>
  

• Analysis of higher-order protein structure and molecular interactions with HDX-MS

  Hydrogen/deuterium exchange mass spectrometry (HDX-MS) for analysing non-covalent structures and interactions of proteins, protein-complexes and drug ligand interaction.
  >>
  

• Quantitative analysis of therapeutic proteins

  Development of mass spectrometry methods for stability, toxicology, pharmacokinetic and pharmacodynamics studies of peptides and proteins in complex biological fluids and tissues.
  >>
  

• Development of new methods for analysis of peptides and proteins by mass spectrometry

  Development of novel MS methods, separation techniques and instrumental setups for analysing peptides and proteins of therapeutic interest in greater details and at higher sensitivity.
  >>
  

• Analysis of the conformation and interactions of membrane proteins

  Studying the topology and conformation of elusive membrane proteins in the lipid bilayer membrane of the cell, including changes to membrane protein conformation during function for instance activation, ligand binding and/or transport of substrate molecules across the cell membrane.
  >>