Protein Analysis Group publishes in Analytical Chemistry
The paper “Improving the Sequence Coverage of Integral Membrane Proteins during Hydrogen/Deuterium Exchange Mass Spectrometry Experiments” was recently published in ACS Analytical Chemistry.
In this work, the Protein Analysis Group collaborated with the group of Claus J. Løland at the Department of Neuroscience (University of Copenhagen), and the group of Petr Man at BioCeV at Institute of Microbiology (Czech Academy of Science).
The conformational dynamics of integral membrane proteins (IMPs) is accessible by HDX-MS but studies often suffer from limited peptide identifications and/or sequence coverage. In this work, we identify experimental parameters that can help improve the digestion of integral membrane proteins in HDX-MS workflows. Our results highlight that susceptibility to proteolytic digestion is highly protein-dependent and that it is important to screen multiple proteases and quench buffer additives when optimizing HDX-MS workflows. We would like to thank everyone involved in the project. This paper highlights some of the important optimization work that lead to the three other recent papers from the group; Merkle et al. (2018), Möller et al., (2019), and Nielsen et al. (2019).