Employees – University of Copenhagen

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Department of Pharmacy > Employees

Hanne Mørck Nielsen

Hanne Mørck Nielsen

Professor

Primary fields of research

Drug formulation and delivery of therapeutic peptides/proteins and oligonucleotides especially focussing on studying the interaction with biological membranes related to delivery of the drug. The influence of the expected (inactive) excipients used in advanced drug delivery systems as well as the drug formulation processing parameters on the toxicity and drug targeting to and interaction with, uptake and transport across the biological membranes and intracellular processing are the primary focus and related to the (physical and chemical) stability of the therapeutic drug. A mechanistic approach to understanding the challenges of particulate formulation development for delivery of biopharmaceuticals is the key point of the research. Present projects comprise research in particulate systems as well as the use of (membrane interacting) peptides (such as antimicrobial peptides and cell-penetrating peptides), polymers and lipids for cellular delivery enhancement of biopharmaceuticals investigating the (potential targeting) effect and mechanisms leading to a desired response. For mechanistic assessment purposes, suitable cell culture models and in vivo models are applied.

 

Teaching

Main teaching activities:

Drug Delviery course (co-organizer), PhD course

Formulation and Delivery course (MIND course)

Advanced Drug Formulation (master course)

Pharmaceutical formulation of peptides and proteins (master course)

Bachelor, master thesis, and individual unit projects

 

Supervision
Master theses 2013-2014

 

Current research

Projects

COMPACT (http://www.imi.europa.eu/content/compact)

DanCARD (http://www.dancardproject.dk/)

IPN (http://hoejteknologifonden.dk/projektgalleri/projektgalleri/millioner_af_infektioner_paa_sygehuse_kan_forhindres/)

 

PhD students

Non-injectable delivery of modified biopharmaceuticals (PhD student Mie Kristensen, 2011-)

Influence of mucosal membrane barrier properties on transport kinetics of oral peptide and protein delivery (Marie Bøgh, 2010 -)

Improved intracellular delivery of antimicrobial peptides by nanomedicine (PhD student Jorrit Water, 2011-)

Impregnation of antimicrobial peptides into a novel composite material - optimizing the interplay between formulation, peptide and material (PhD student Søren Langer Steffensen, 2011-)

A mechanistic approach of membrane interactions of antimicrobial peptides (Jesper Søborg Bahnsen, 2011-)

 

Selected publications

  1. Published

    Trehalose preserves DDA/TDB liposomes and their adjuvant effect during freeze-drying

    Christensen, D., Foged, C., Rosenkrands, I., Nielsen, H. M., Andersen, P. & Agger, E. M. 2007 In : Biochimica et Biophysica Acta. 1768, 9, p. 2120-2129

    Publication: Research - peer-reviewJournal article

  2. Published

    Cell-penetrating peptides for drug delivery across membrane barriers

    Foged, C. & Nielsen, H. M. 2008 In : Expert Opinion on Drug Delivery. 5, 1, p. 105-17 12 p.

    Publication: ResearchReview

  3. Published

    Phospholipase A2 sensitive liposomes for delivery of small interfering RNA (siRNA)

    Foged, C., Nielsen, H. M. & Frøkjær, S. 2007 In : Journal of Liposome Research. 17, 3-4

    Publication: Research - peer-reviewJournal article

  4. Published

    Liposomes for phospholipase A2 triggered siRNA release: preparation and in vitro test

    Foged, C., Nielsen, H. M. & Frøkjær, S. 2007 In : Internation Journal of Pharmaceutics. 331, 2, p. 160-166

    Publication: Research - peer-reviewJournal article

  5. Published

    Farvel til sprøjten: udvikling af vaccineplaster med nanopartikler

    Madsen, H. B., Ifversen, P., Madsen, F., Manniche, S. & Nielsen, H. M. 2007 In : Lægemiddelforskning. p. 12-13

    Publication: CommunicationJournal article

  6. Published

    Polymeric nanocarriers for siRNA delivery: challenges and future prospects

    Cun, D., Jensen, L. B., Nielsen, H. M., Moghimi, S. M. & Foged, C. 2008 In : Journal of Biomedical Nanotechnology. 4, 3, p. 258-275

    Publication: ResearchReview

  7. Published

    Spray drying of siRNA-containing PLGA nanoparticles intended for inhalation

    Jensen, D. M. K., Cun, D., Maltesen, M. J., Frøkjær, S., Nielsen, H. M. & Foged, C. 2010 In : Journal of Controlled Release. 142, p. 138-145

    Publication: Research - peer-reviewJournal article

  8. Published

    Design of an inhalable dry powder formulation of DOTAP-modified PLGA nanoparticles loaded with siRNA

    Jensen, D. M. K., Jensen, L. B., Koocheki, S., Cun, D., Nielsen, H. M., Foged, C. & Bengtson, L. 2012 In : Journal of Controlled Release. 157, 1, p. 141-148

    Publication: Research - peer-reviewJournal article

  9. Published

    Preparation and characterization of poly (DL-lactide-co-glycolide) nanoparticles for siRNA delivery

    Cun, D., Foged, C., Yang, M., Frøkjær, S. & Nielsen, H. M. 2010 In : Internation Journal of Pharmaceutics. 390, 1, p. 70-75

    Publication: Research - peer-reviewJournal article

  10. Published

    High loading efficiency and sustained release of siRNA encapsulated in PLGA nanoparticles: quality by design optimization and characterization

    Cun, D., Jensen, D. M. K., Maltesen, M. J., Bunker, M., Whitside, P., Scurr, D., Foged, C. & Nielsen, H. M. 2011 In : European Journal of Pharmaceutics and Biopharmaceutics. 77, 1, p. 26-35

    Publication: Research - peer-reviewJournal article

  11. Published

    a, a´- trehalose 6,6´- dibehenate in non-phospholipid-based liposomes enables direct interaction with trehalose, offering stability during freeze-drying

    Christensen, D., Kirby, D., Foged, C., Agger, E. M., Andersen, P., Perrie, Y. & Nielsen, H. M. 2008 In : Biochimica et Biophysica Acta - Biomembranes. 1778, 5, p. 1365-1373

    Publication: Research - peer-reviewJournal article

  12. Published

    Dendritiske nanostrukturer som transportører af genestisk medicin

    Jensen, L. B., Nielsen, H. M. & Foged, C. 2008 In : Lægemiddelforskning. p. 13-15

    Publication: CommunicationJournal article

  13. Published

    Cellular uptake and membrane-destabilising properties of a-peptide/ß-peptoid chimeras: lessons for the design of new cell-penetrating peptides

    Foged, C., Franzyk, H., Bahrami, S., Frøkjær, S., Jaroszewski, J. W., Nielsen, H. M. & Olsen, C. A. 2008 In : Biochimica et Biophysica Acta. 1778, 11, p. 2487-2495

    Publication: Research - peer-reviewJournal article

  14. Published

    Status and future prospects of lipid-based particulate delivery systems as vaccine adjuvants and their combination with immunostimulators

    Nordly, P. J., Madsen, H. B., Nielsen, H. M. & Foged, C. 2009 In : Expert Opinion on Drug Delivery. 6, 7, p. 1-16

    Publication: ResearchReview

  15. Published

    CAF01 liposomes as a mucosal vaccine adjuvant: In vitro and in vivo investigations

    Christensen, D., Foged, C., Rosenkrands, I., Lundberg, C. V., Andersen, P., Agger, E. M. & Nielsen, H. M. 2010 In : Internation Journal of Pharmaceutics. 390, p. 19-24

    Publication: Research - peer-reviewJournal article

  16. Published

    NIR transmission spectroscopy for rapid determination of lipid and lyoprotector content in liposomal vaccine adjuvant system CAF01

    Christensen, D., Allesø, M., Rosenkrands, I., Rantanen, J. T., Foged, C., Agger, E. M., Andersen, P. & Nielsen, H. M. 2008 In : European Journal of Pharmaceutics and Biopharmaceutics. 70, 3, p. 914-920

    Publication: Research - peer-reviewJournal article

  17. Published

    Cell-penetrating peptides for cytosolic delivery of biomacromolecules

    Foged, C., Jing, X. & Nielsen, H. M. 2010 Organelle-specific pharmaceutical nanotechnology. Weissig, V. & D'Souza, G. G. M. (eds.). Hoboken: John Wiley & Sons Ltd, p. 403-432

    Publication: ResearchBook chapter

  18. Published

    Modulation of the adjuvant activity of DDA/TDB liposomes with immunostimulators

    Nordly, P. J., Foged, C., Christensen, D., Lindenstrøm, T., Nielsen, H. M. & Agger, E. 2010

    Publication: ResearchPoster

  19. Published

    Incorporation of a synthetic mycobacterial monomycoloyl glycerol analogue stabilizes dimethyldioctadecylammonium liposomes and potentiates their adjuvant effect in vivo

    Nordly, P. J., Korsholm, K. S., Pedersen, E. A., Khilji, T. S., Franzyk, H., Jørgensen, L., Nielsen, H. M., Agger, E. M. & Foged, C. 2011 In : European Journal of Pharmaceutics and Biopharmaceutics. 77, 1, p. 89-98

    Publication: Research - peer-reviewJournal article

  20. Published

    Stabilization of liposomes during drying

    Ingvarsson, P. Þ., Yang, M., Nielsen, H. M., Rantanen, J. T. & Foged, C. 2011 In : Expert Opinion on Drug Delivery. 8, 3, p. 375-388

    Publication: ResearchReview

  21. Published

    A synthetic mycobacterial monomycoloyl glycerol analogue stabilizes DDA liposomes

    Nordly, P. J., Pedersen, E. A., Khiljii, T. S., Agger, E. M., Jørgensen, L., Nielsen, H. M., Korsholm, K. S., Franzyk, H. & Foged, C. 2010 In : CRS Newsletter. 27, 2, p. 12 14 p.

    Publication: ResearchJournal article

  22. Published

    Incorporation of the TLR4 agonist monophosphoryl lipid A into the bilayer of DDA/DTDB liposomes : physico-chemical characterization and induction of CD8+ T-Cell responses in vivo

    Nordly, P. J., Agger, E. M., Andersen, P., Nielsen, H. M. & Foged, C. 2011 In : Pharmaceutical Research. 28, 3, p. 553-562

    Publication: Research - peer-reviewJournal article

  23. Published

    Molecular characterization of the interaction between siRNA and PAMAM G7 dendrimers by SAXS, ITC, and molecular dynamics simultations

    Jensen, L. B., Mortensen, K., Pavan, G. M., Kasimova, M. R., Jensen, D. K., Gadzhyeva, V., Nielsen, H. M. & Foged, C. 2010 In : Biomacromolecules. 11, 12, p. 3571-3577

    Publication: Research - peer-reviewJournal article

  24. Published

    Elucidating the molecular mechanism of PAMAM-siRNA dendriplex self-assembly: Effect of dendrimer charge density

    Jensen, L. B., Pavan, G. M., Kasimova, M. R., Rutherford, S., Danani, A., Nielsen, H. M. & Foged, C. 2011 In : International Journal of Pharmaceutics. 416, p. 410-418

    Publication: Research - peer-reviewJournal article

  25. Published

    Immunity by formulation design: induction of high CD8+ T-cell responses by poly (I:C) incorporated into the CAF01 adjuvant via a double emulsion method

    Nordly, P. J., Rose, F., Christensen, D., Nielsen, H. M., Andersen, P., Agger, E. M. & Foged, C. 2011 In : Journal of Controlled Release. 150, 3, p. 307-317

    Publication: Research - peer-reviewJournal article

  26. Published

    Comparison of polymeric siRNA nanocarriers in murine LPS-activated macrophage cell line: Gene silencing, toxicity and off-target gene expression

    Jensen, L. B., Griger, J., Naeye, B., Varkouhi, A. K., Raemdonck, K., Schiffelers, R., Lammers, T., Storm, G., Smedt, S. C. D., Sproat, B. S., Nielsen, H. M. & Foged, C. 2012 In : Pharmaceutical Research. 29, p. 669-682

    Publication: Research - peer-reviewJournal article

  27. Published

    MRI-assessed therapeutic effects of locally administered PLGA nanoparticles loaded with anti-inflammatory siRNA in a murine arthritis model

    Te Boekhorst, B. C. M., Jensen, L. B., Colombo, S., Varkouhi, A. K., Schiffelers, R. M., Lammers, T., Storm, G., Nielsen, H. M., Strijkers, G. J., Foged, C. & Nicolay, K. 2012 In : Journal of controlled release : official journal of the Controlled Release Society. 161, 3, p. 772-80 9 p.

    Publication: Research - peer-reviewJournal article

  28. Published

    Microcalorimetric and spectroscopic studies on the mechanism of interaction between novel peptidomimetics and lipid bilayers

    Nielsen, H. M., Jing, X., Simonsen, A., Kasimova, M. R., Malmsten, M., Franzyk, H. & Foged, C. 2010 In : Journal of Peptide Science. 16, s1, p. 151 1 p.

    Publication: ResearchConference abstract in journal

  29. Published

    Microcalorimetric and spectroscopic studies on the mechanism of interaction between novel peptoids and lipid bilayers - effect of length, charge and N-terminal end group

    Jing, X., Simonsen, A. H., Kasimova, M. R., Franzyk, H., Foged, C. & Nielsen, H. M. 2010 In : Drug Discovery Today. 15, 23/24, p. 1109

    Publication: ResearchConference abstract in journal

  30. Published

    Innovativt nanopartikeldesign bag skræddersyede vacciner

    Nordly, P. J., Agger, E. M., Nielsen, H. M. & Foged, C. 2009 In : Lægemiddelforskning. p. 19-21

    Publication: ResearchJournal article

  31. Published

    Metabolic cleavage and translocation efficiency of selected cell penetrating peptides: A comparative study with epithelial cell cultures

    Foerg, C., Weller, K. M., Rechsteiner, H., Nielsen, H. M., Fernandez-Carneado, J., Brunisholz, R., Giralt, E. & Merkle, H. P. 2008 In : AAPS Journal. 10, 2, p. 349-359

    Publication: Research - peer-reviewJournal article

  32. Published

    In Vitro cutaneous application of ISCOMs on human skin enhances delivery of hydrophobic model compounds through the stratum corneum

    Madsen, H. B., Ifversen, P., Madsen, F., Larsen, B. B., Hausser, I. & Nielsen, H. M. 2009 In : AAPS Journal. 11, 4, p. 728-739

    Publication: Research - peer-reviewJournal article

  33. Published

    Biotechnology-based pharmaceuticals

    Jørgensen, L., Nielsen, H. M. & Frøkjær, S. 2009 Modern pharmaceutics: applications and advances. Florence, A. T. & Siepmann, J. (eds.). 5 ed. New York: Informa Healthcare, Vol. 2, p. 259-293 (Drugs and the pharmaceutical sciences, Vol. 188).

    Publication: ResearchBook chapter

  34. Published

    Influence of skin barrier on in-vitro penetration properties of solid lipid nanoparticles

    Jensen, L. B., Nielsen, H. M. & Petersson, K. 2010 In : Journal of Pharmacy and Pharmacology. p. 793-794

    Publication: ResearchConference abstract in journal

  35. Published

    Antimicrobial, hemolytic, and cytotoxic activities of ß-peptoid-peptide hybrid oligomers: Improved properties compared to natural AMPs

    Olsen, C. A., Ziegler, H. L., Nielsen, H. M., Frimodt-Møller, N., Jaroszewski, J. W. & Franzyk, H. 2010 In : ChemBioChem. 11, 10, p. 1356-1360

    Publication: Research - peer-reviewJournal article

  36. Published

    Delivery technologies for biopharmaceuticals: Peptides, proteins, nucleic acids and vaccines

    Jørgensen, L. & Nielsen, H. M. 2009 John Wiley & Sons Ltd. 413 p.

    Publication: Research - peer-reviewBook

  37. Published

    Corticosteroid solubility and lilpid polarity control release from solid lipid nanoparticles

    Jensen, L. B., Magnusson, E., Gunnarsson, L., Vermehren, C., Nielsen, H. M. & Petersson, K. 2010 In : Internation Journal of Pharmaceutics. 390, 1, p. 53-60

    Publication: Research - peer-reviewJournal article

  38. Published

    Cellular and intracellular distribution of recombinant activated factor VII in the rat liver

    Johansen, T. S., Nielsen, H. M., Christensen, E. I. & Appa, R. S. 2010 In : Thrombosis and Haemostasis. 103, 4, p. 860-862

    Publication: Research - peer-reviewJournal article

  39. Published

    On the temperature dependence of complex formation between chitosan and proteins

    Kasimova, M. R., Valázquez-Campoy, A. & Nielsen, H. M. 2011 In : Biomacromolecules. 12, 7, p. 2534-2543

    Publication: Research - peer-reviewJournal article

  40. Published

    The unsialylated subpopulation of recombinant activated factor VII binds to the asialo-glycoprotein receptor (ASGPR) on primary rat hepatocytes

    Johansen, T. S., Nielsen, H. M., Christensen, E. I. & Appa, R. S. 2010 In : Thrombosis and Haemostasis. 104, 6, p. 1166-1173

    Publication: Research - peer-reviewJournal article

  41. Published

    In vivo clearance and metabolism of recombinant activated factor VII (rFVIIa) and its complexes with plasma protease inhibitors in the liver

    Johansen, T. S., Appa, R. S., Christensen, E. I., Ioannou, Y. A., Krogh, T. N., Karpf, D. M. & Nielsen, H. M. 2011 In : Thrombosis Research. 127, 4, p. 356-362

    Publication: Research - peer-reviewJournal article

  42. Published

    Biodegradable nanocomposite microparticles as drug delivering injectable cell scaffolds

    Wen, Y., Gallego, M. R., Nielsen, L. F., Jørgensen, L., Everland, H., Møller, E. H. & Nielsen, H. M. 2011 In : Journal of Controlled Release. 156, 1, p. 11-20

    Publication: Research - peer-reviewJournal article

  43. Published

    Issues in development of biodrug delivery systems

    Jørgensen, L., Frøkjær, S. & Nielsen, H. M. 2010 Biodrug delivery systems: fundamentals, applications and clinical development. Morishita, M. & Park, K. (eds.). New York: Informa Healthcare, p. 13-25

    Publication: ResearchBook chapter

  44. Published

    Investigation of the interaction between modified ISCOMs and stratum corneum lipid model systems

    Madsen, H. B., Arboe-Andersen, H. M., Rozlosnik, N., Madsen, F., Ifversen, P., Kasimova, M. R. & Nielsen, H. M. 2010 In : Biochimica et Biophysica Acta - Biomembranes. 1798, p. 1779-1789

    Publication: Research - peer-reviewJournal article

  45. Published

    Cyclodextrins: efficient biocompatible solubilizing excipients for bromhexine liquid and semi-solid drug delivery systems

    Stojanov, M., Nielsen, H. M. & Larsen, K. L. 2012 In : International Journal of Pharmaceutics. 422, 1-2, p. 349-355

    Publication: Research - peer-reviewJournal article

  46. Published

    Delivery of dermatan sulfate from polyelectrolyte complex-containing alginate composite microspheres for tissue regeneration

    Wen, Y., Grøndahl, L., Gallego, M. R., Jørgensen, L., Møller, E. H. & Nielsen, H. M. 2012 In : Biomacromolecules. 13, p. 905-917

    Publication: Research - peer-reviewJournal article

  47. Published

    Structure of immune stimulating complex matrices and immune stimulating complexes in suspension determined by small-angle X-ray scattering

    Pedersen, J. S., Oliveira, C. L. P. D., Madsen, H. B., Arleth, L., Manniche, S., Kirkby, N. & Nielsen, H. M. 2012 In : Biophysical Journal. 102, 10, p. 2372-2380

    Publication: Research - peer-reviewJournal article

  48. Published

    In vitro placental model optimization for nanoparticle transport studies

    Cartwright, L., Poulsen, M. S., Nielsen, H. M., Pojana, G., Knudsen, L. E., Saunders, M. & Rytting, E. 2012 In : International Journal of Nanomedicine (Online). 7, p. 497-510

    Publication: Research - peer-reviewJournal article

  49. Published

    Studies towards improved cell-penetrating peptide-promoted macromolecular drug delivery

    Dahlqvist Clausen, J. A., Linderoth, L., Andersen, R. B., Franzyk, H. & Nielsen, H. M. 2010 In : Drug Discovery Today. 15, 23-24, p. 1109 1 p.

    Publication: ResearchConference abstract in journal

  50. Published

    In vitro penetration properties of solid lipid nanoparticles in intact and barrier-impaired skin

    Jensen, L. B., Petersson, K. & Nielsen, H. M. 2011 In : European Journal of Pharmaceutics and Biopharmaceutics. 79, 1, p. 68-75

    Publication: Research - peer-reviewJournal article

  51. Published

    Antimicrobial and cell-penetrating properties of penetratin analogs: effect of sequence and secondary structure

    Bahnsen, J. S., Franzyk, H., Sandberg-Schaal, A. & Nielsen, H. M. 2013 In : B B A - Biomembranes. 1828, 2, p. 223-232 10 p.

    Publication: Research - peer-reviewJournal article

  52. Published

    Solid-phase route to Fmoc-protected cationic amino acid building blocks

    Clausen, J. D., Linderoth, L., Nielsen, H. M. & Franzyk, H. 2012 In : Amino Acids. 43, 4, p. 1633-41 9 p.

    Publication: Research - peer-reviewJournal article

  53. Published

    Studies towards improved stability and permeability of a cell-penetrating peptide

    Dahlqvist Clausen, J. A., Linderoth, L., Andersen, R. B., Franzyk, H. & Nielsen, H. M. 2010 In : Journal of Peptide Science. 16, p. 148 1 p.

    Publication: ResearchConference abstract in journal

  54. Published

    Kan nanopartikler bruges i behandlingen af hudsygdomme?

    Jensen, L. B., Knudsen, N. Ø., Foged, C. & Nielsen, H. M. 2010 In : Lægemiddelforskning. 2010, p. 10-12

    Publication: CommunicationJournal article

  55. Published

    Genetisk nanomedicin til behandling af leddegigt

    Foged, C., Jensen, L. B. & Nielsen, H. M. Dec 2011 In : Lægemiddelforskning. p. 7-9

    Publication: CommunicationJournal article

  56. Published

    Gendannelse af ødelagt væv med stamceller

    Møller, E. H., Wen, Y. & Nielsen, H. M. Dec 2011 In : Lægemiddelforskning. 2011, p. 13-15

    Publication: CommunicationJournal article

ID: 1299091