Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses
Research output: Contribution to journal › Journal article › peer-review
To improve the sample handling, and reduce cost and preparation time, of peptide mapping LC-MS workflows in protein analytical research, we here investigate the possibility of replacing conventional enzymatic digestion methods with a polymer microfluidic chip based enzyme reactor. Off-stoichiometric thiol-ene is utilized as both bulk material and as a monolithic stationary phase for immobilization of the proteolytic enzyme pepsin. The digestion efficiency of the, thiol-ene based, immobilized enzyme reactor (IMER) is compared to that of a conventional, agarose packed bed, pepsin IMER column commonly used in LC-MS based protein analyses. The chip IMER is found to rival the conventional column in terms of digestion efficiency at comparable residence time and, using a 3D-printed interface, be directly interfaceable with LC-MS.
Original language | English |
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Journal | Analytical Chemistry |
Volume | 89 |
Issue number | 8 |
Pages (from-to) | 4573-4580 |
Number of pages | 8 |
ISSN | 0003-2700 |
DOIs | |
Publication status | Published - 21 Mar 2017 |
- Journal Article
Research areas
ID: 176596936