The T-Cell Receptor Can Bind to the Peptide-Bound Major Histocompatibility Complex and Uncomplexed β2-Microglobulin through Distinct Binding Sites

Research output: Contribution to journalJournal articleResearchpeer-review

  • Patrick S Merkle
  • Melita Irving
  • Song Hongjian
  • Mathias Ferber
  • Thomas J D Jørgensen
  • Kirsten Scholten
  • Immanuel Luescher
  • George Coukos
  • Vincent Zoete
  • Michel A Cuendet
  • Olivier Michielin
  • Rand, Kasper Dyrberg

T-Cell receptor (TCR)-mediated recognition of the peptide-bound major histocompatibility complex (pMHC) initiates an adaptive immune response against antigen-presenting target cells. The recognition events take place at the TCR-pMHC interface, and their effects on TCR conformation and dynamics are controversial. Here, we have measured the time-resolved hydrogen/deuterium exchange (HDX) of a soluble TCR in the presence and absence of its cognate pMHC by mass spectrometry to delineate the impact of pMHC binding on solution-phase structural dynamics in the TCR. Our results demonstrate that while TCR-pMHC complex formation significantly stabilizes distinct CDR loops of the TCR, it does not trigger structural changes in receptor segments remote from the binding interface. Intriguingly, our HDX measurements reveal that the TCR α-constant domain (C- and F-strand) directly interacts with the unbound MHC light chain, β2-microglobulin (β2m). Surface plasmon resonance measurements corroborated a binding event between TCR and β2m with a dissociation constant of 167 ± 20 μM. We propose a model structure for the TCR-β2m complex based on a refined protein-protein docking approach driven by HDX data and information from molecular dynamics simulations. Using a biological assay based on TCR gene-engineered primary human T cells, we did not observe a significant effect of β2m on T-cell cytotoxicity, suggesting an alternate role for β2m binding. Overall, we show that binding of β2m to the TCR occurs in vitro and, as such, not only should be considered in structure-function studies of the TCR-pMHC complex but also could play a hitherto unidentified role in T-cell function in vivo.

Original languageEnglish
JournalBiochemistry
Volume56
Issue number30
Pages (from-to)3945-3961
Number of pages17
ISSN0006-2960
DOIs
Publication statusPublished - 1 Aug 2017

    Research areas

  • Amino Acid Substitution, Binding Sites, Cells, Cultured, Cytotoxicity, Immunologic, Deuterium Exchange Measurement, Humans, Kinetics, Ligands, Major Histocompatibility Complex, Models, Molecular, Molecular Docking Simulation, Molecular Dynamics Simulation, Mutation, Peptide Fragments, Protein Conformation, Protein Engineering, Protein Interaction Domains and Motifs, Protein Stability, Receptors, Antigen, T-Cell, alpha-beta, Recombinant Proteins, T-Lymphocytes, beta 2-Microglobulin, Journal Article

ID: 185404726