Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats

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Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats. / Jensen, Anna Guldvang; Hansen, Cecilie Hurup; Weisser, Johan J; Halling-Sørensen, Bent; Styrishave, Bjarne.

In: Reproductive Toxicology, Vol. 58, 12.2015, p. 174-83.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jensen, AG, Hansen, CH, Weisser, JJ, Halling-Sørensen, B & Styrishave, B 2015, 'Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats', Reproductive Toxicology, vol. 58, pp. 174-83. https://doi.org/10.1016/j.reprotox.2015.10.005

APA

Jensen, A. G., Hansen, C. H., Weisser, J. J., Halling-Sørensen, B., & Styrishave, B. (2015). Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats. Reproductive Toxicology, 58, 174-83. https://doi.org/10.1016/j.reprotox.2015.10.005

Vancouver

Jensen AG, Hansen CH, Weisser JJ, Halling-Sørensen B, Styrishave B. Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats. Reproductive Toxicology. 2015 Dec;58:174-83. https://doi.org/10.1016/j.reprotox.2015.10.005

Author

Jensen, Anna Guldvang ; Hansen, Cecilie Hurup ; Weisser, Johan J ; Halling-Sørensen, Bent ; Styrishave, Bjarne. / Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats. In: Reproductive Toxicology. 2015 ; Vol. 58. pp. 174-83.

Bibtex

@article{f145ab69085c494bac048d83849a01d9,

title = "Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats",

abstract = "Endocrine modulating effects of Simvastatin (SV) and its metabolite, Simvastatin β-hydroxy acid (SVA), were investigated in H295R cells and in female Sprague-Dawley (SPRD) rats. H295R cells were exposed to SV and SVA concentrations from 0 to 10μM for 48h. Four groups of SPRD rats received 0 (CT), 1.3 (L), 5.0 (M), and 20.0 (H)mg SV/kg bw/day for 14 days. 10 Steroids were investigated in H295R growth media, and in tissues and plasma from rats using GC-MS/MS. Plasma LH and FSH were quantified by ELISA. In the H295R assay, SV and SVA particularly decreased progestagens with IC50-values from 0.10-0.13μM for SV and from 0.019-0.055μM for SVA. In rats, SV decreased progestagens in ovaries, brain and plasma, and plasma FSH in the M (72.4% decrease) and H group (76.6% decrease). Because progestagens and gonadotropins are major players in fertility, administration of SV might exert negative effects on female reproduction.",

author = "Jensen, {Anna Guldvang} and Hansen, {Cecilie Hurup} and Weisser, {Johan J} and Bent Halling-S{\o}rensen and Bjarne Styrishave",

year = "2015",

month = dec,

doi = "10.1016/j.reprotox.2015.10.005",

language = "English",

volume = "58",

pages = "174--83",

journal = "Reproductive Toxicology",

issn = "0890-6238",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats

AU - Jensen, Anna Guldvang

AU - Hansen, Cecilie Hurup

AU - Weisser, Johan J

AU - Halling-Sørensen, Bent

AU - Styrishave, Bjarne

PY - 2015/12

Y1 - 2015/12

N2 - Endocrine modulating effects of Simvastatin (SV) and its metabolite, Simvastatin β-hydroxy acid (SVA), were investigated in H295R cells and in female Sprague-Dawley (SPRD) rats. H295R cells were exposed to SV and SVA concentrations from 0 to 10μM for 48h. Four groups of SPRD rats received 0 (CT), 1.3 (L), 5.0 (M), and 20.0 (H)mg SV/kg bw/day for 14 days. 10 Steroids were investigated in H295R growth media, and in tissues and plasma from rats using GC-MS/MS. Plasma LH and FSH were quantified by ELISA. In the H295R assay, SV and SVA particularly decreased progestagens with IC50-values from 0.10-0.13μM for SV and from 0.019-0.055μM for SVA. In rats, SV decreased progestagens in ovaries, brain and plasma, and plasma FSH in the M (72.4% decrease) and H group (76.6% decrease). Because progestagens and gonadotropins are major players in fertility, administration of SV might exert negative effects on female reproduction.

AB - Endocrine modulating effects of Simvastatin (SV) and its metabolite, Simvastatin β-hydroxy acid (SVA), were investigated in H295R cells and in female Sprague-Dawley (SPRD) rats. H295R cells were exposed to SV and SVA concentrations from 0 to 10μM for 48h. Four groups of SPRD rats received 0 (CT), 1.3 (L), 5.0 (M), and 20.0 (H)mg SV/kg bw/day for 14 days. 10 Steroids were investigated in H295R growth media, and in tissues and plasma from rats using GC-MS/MS. Plasma LH and FSH were quantified by ELISA. In the H295R assay, SV and SVA particularly decreased progestagens with IC50-values from 0.10-0.13μM for SV and from 0.019-0.055μM for SVA. In rats, SV decreased progestagens in ovaries, brain and plasma, and plasma FSH in the M (72.4% decrease) and H group (76.6% decrease). Because progestagens and gonadotropins are major players in fertility, administration of SV might exert negative effects on female reproduction.

U2 - 10.1016/j.reprotox.2015.10.005

DO - 10.1016/j.reprotox.2015.10.005

M3 - Journal article

C2 - 26476359

VL - 58

SP - 174

EP - 183

JO - Reproductive Toxicology

JF - Reproductive Toxicology

SN - 0890-6238

ER -

ID: 161588544

Department of Pharmacy