Longevity of elastin in human intervertebral disc as probed by the racemization of aspartic acid
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Longevity of elastin in human intervertebral disc as probed by the racemization of aspartic acid. / Sivan, Sarit-Sara; Van El, Benno; Merkher, Yulia; Schmelzer, Christian E H; Zuurmond, Anne-Marie; Heinz, Andrea; Wachtel, Ellen; Varga, Peter-Paul; Lazary, Aron; Brayda-Bruno, Marco; Maroudas, Alice.
In: B B A - Reviews on Cancer, Vol. 1820, No. 10, 10.2012, p. 1671-7.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Longevity of elastin in human intervertebral disc as probed by the racemization of aspartic acid
AU - Sivan, Sarit-Sara
AU - Van El, Benno
AU - Merkher, Yulia
AU - Schmelzer, Christian E H
AU - Zuurmond, Anne-Marie
AU - Heinz, Andrea
AU - Wachtel, Ellen
AU - Varga, Peter-Paul
AU - Lazary, Aron
AU - Brayda-Bruno, Marco
AU - Maroudas, Alice
N1 - Copyright © 2012 Elsevier B.V. All rights reserved.
PY - 2012/10
Y1 - 2012/10
N2 - BACKGROUND: Aging and degeneration of human intervertebral disc (IVD) are associated with biochemical changes, including racemization and glycation. These changes can only be counteracted by protein turnover. Little is known about the longevity of IVD elastin in health or disease. Yet, such knowledge is important for a quantitative understanding of tissue synthesis and degradation.METHODS: We have measured the accumulation of d-Asp and pentosidine in IVD elastin. Samples representing a broad range of ages (28-82years) and degeneration grades (1-5) were analyzed.RESULTS: d/l-Asp for elastin increased linearly with age from 3.2% (early 30s) to 14.8% (early 80s) for normal tissue (grades 1-2) and from 1.7% (late 20s) to 6.0% (until the mid 50s) for degenerate tissue (grades 3-5) with accumulation rates of 16.2±3.1×10(-4) and 11.7±3.8×10(-4)year(-1), respectively; no significant difference was found between these values (p<0.05). Above the mid 50s, a decrease in d-Asp accumulation was recorded in the degenerate tissue. d-Asp accumulation correlated well with pentosidine content for elastin from healthy and degenerate tissues combined. We conclude that IVD elastin is metabolically-stable and long-lived in both healthy and degenerate human IVDs, with signs of new synthesis in the latter. The correlation of d-Asp with pentosidine content suggests that both these agents may be used as markers in the overall aging process of IVD.GENERAL SIGNIFICANCE: Accumulation of modified IVD elastin argues for its longevity and may have a negative impact on its role in disc function. Weak signs of newly synthesized molecules may act to counteract this effect in degenerate tissue.
AB - BACKGROUND: Aging and degeneration of human intervertebral disc (IVD) are associated with biochemical changes, including racemization and glycation. These changes can only be counteracted by protein turnover. Little is known about the longevity of IVD elastin in health or disease. Yet, such knowledge is important for a quantitative understanding of tissue synthesis and degradation.METHODS: We have measured the accumulation of d-Asp and pentosidine in IVD elastin. Samples representing a broad range of ages (28-82years) and degeneration grades (1-5) were analyzed.RESULTS: d/l-Asp for elastin increased linearly with age from 3.2% (early 30s) to 14.8% (early 80s) for normal tissue (grades 1-2) and from 1.7% (late 20s) to 6.0% (until the mid 50s) for degenerate tissue (grades 3-5) with accumulation rates of 16.2±3.1×10(-4) and 11.7±3.8×10(-4)year(-1), respectively; no significant difference was found between these values (p<0.05). Above the mid 50s, a decrease in d-Asp accumulation was recorded in the degenerate tissue. d-Asp accumulation correlated well with pentosidine content for elastin from healthy and degenerate tissues combined. We conclude that IVD elastin is metabolically-stable and long-lived in both healthy and degenerate human IVDs, with signs of new synthesis in the latter. The correlation of d-Asp with pentosidine content suggests that both these agents may be used as markers in the overall aging process of IVD.GENERAL SIGNIFICANCE: Accumulation of modified IVD elastin argues for its longevity and may have a negative impact on its role in disc function. Weak signs of newly synthesized molecules may act to counteract this effect in degenerate tissue.
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Aging
KW - Amino Acid Isomerases
KW - Aspartic Acid
KW - Autopsy
KW - Elastin
KW - Female
KW - Humans
KW - Intervertebral Disc
KW - Intervertebral Disc Degeneration
KW - Longevity
KW - Male
KW - Middle Aged
KW - Molecular Probe Techniques
KW - Time Factors
KW - Comparative Study
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1016/j.bbagen.2012.06.010
DO - 10.1016/j.bbagen.2012.06.010
M3 - Journal article
C2 - 22728886
VL - 1820
SP - 1671
EP - 1677
JO - Biochimica et Biophysica Acta - Reviews on Cancer
JF - Biochimica et Biophysica Acta - Reviews on Cancer
SN - 0304-419X
IS - 10
ER -
ID: 186422239