TY - JOUR

T1 - Interaction of dendritic cells with antigen-containing liposomes: effect of bilayer composition

AU - Foged, Camilla

AU - Arigita, Carmen

AU - Sundblad, Anne

AU - Jiskoot, Wim

AU - Storm, Gert

AU - Frøkjær, Sven

N1 - Keywords: Animals; Antigens; Cells, Cultured; Dendritic Cells; Drug Carriers; Flow Cytometry; Fluorescent Antibody Technique; Humans; Lipid Bilayers; Liposomes; Mannose; Mice; Mice, Inbred BALB C; Microscopy, Confocal; Monocytes; Particle Size; Subcellular Fractions

PY - 2004

Y1 - 2004

N2 - Vaccine efficacy might be improved by exploiting the potent antigen presenting properties of dendrite cells (DCs), since their ability to stimulate specific major histocompatibility complex-restricted immune responses has been well documented during the recent years. In that light, we investigated how the interaction of antigen-containing liposomes with DCs was affected by the bilayer composition. Monocyte-derived human DCs and murine bone marrow-derived DCs were analysed and compared upon in vitro incubation with liposomes by flow cytometry and confocal microscopy. Anionic liposomes with a bilayer composition of phosphatidylcholine, cholesterol and phosphatidylglycerol or phosphatidylserine interacted with a limited fraction of the total DC population in case of both DC types. Inclusion of mannosylated phosphatidylethanolamine (Man-PE) for targeting to the mannose receptor (MR) increased the interaction of negatively charged liposomes with both human and murine DCs. This increase could be blocked in human DCs by addition of the polysaccharide mannan indicating that uptake might be mediated by the mannose receptor. Cationic liposomes containing trimethyl ammonium propane interacted with a very high percentage of both DC types and could be detected in high amounts intracellularly. In conclusion, liposome bilayer composition has an important effect on interaction with DCs and might be critical for the vaccination outcome.

AB - Vaccine efficacy might be improved by exploiting the potent antigen presenting properties of dendrite cells (DCs), since their ability to stimulate specific major histocompatibility complex-restricted immune responses has been well documented during the recent years. In that light, we investigated how the interaction of antigen-containing liposomes with DCs was affected by the bilayer composition. Monocyte-derived human DCs and murine bone marrow-derived DCs were analysed and compared upon in vitro incubation with liposomes by flow cytometry and confocal microscopy. Anionic liposomes with a bilayer composition of phosphatidylcholine, cholesterol and phosphatidylglycerol or phosphatidylserine interacted with a limited fraction of the total DC population in case of both DC types. Inclusion of mannosylated phosphatidylethanolamine (Man-PE) for targeting to the mannose receptor (MR) increased the interaction of negatively charged liposomes with both human and murine DCs. This increase could be blocked in human DCs by addition of the polysaccharide mannan indicating that uptake might be mediated by the mannose receptor. Cationic liposomes containing trimethyl ammonium propane interacted with a very high percentage of both DC types and could be detected in high amounts intracellularly. In conclusion, liposome bilayer composition has an important effect on interaction with DCs and might be critical for the vaccination outcome.

U2 - 10.1016/j.vaccine.2003.11.008

DO - 10.1016/j.vaccine.2003.11.008

M3 - Journal article

C2 - 15121302

VL - 22

SP - 1903

EP - 1913

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 15-16

ER -